| 葛根素对成骨细胞生物学作用的实验研究 |
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| 引用本文： | 臧洪敏陈君长,刘亦恒,王坤正. 葛根素对成骨细胞生物学作用的实验研究[J]. 中国中药杂志, 2005, 30(24): 1947-1949 |
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| 作者姓名： | 臧洪敏陈君长 刘亦恒 王坤正 |
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| 作者单位： | 西安交通大学,第二医院,陕西,西安,710004 |
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| 摘 要： | 目的:观察葛根素对体外培养SD大鼠成骨细胞增殖、分化、矿化的影响。方法:取新生SD大鼠的颅骨,胶原酶法培养成骨细胞。应用MTT法,对硝基苯磷酸盐法(PNPP)及茜素红染色方法观察葛根素对体外培养成骨细胞的增殖、碱性磷酸酶活性及矿化结节形成的影响。结果:葛根素具有刺激成骨细胞增殖,提高碱性磷酸酶活性及矿化结节形成数量的作用(P<0.01或P<0.05)。结论:葛根素具有促进成骨细胞的增殖、分化及矿化的作用。
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| 关 键 词： | 葛根素 细胞培养 成骨细胞 骨质疏松症 |
| 文章编号： | 1001-5302（2005）24-1947-03 |
| 收稿时间： | 2005-05-28 |
| 修稿时间： | 2005-05-28 |
Experimental study of the effects of puerarin on biological characters of osteoblasts in vitro |
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| ZANG Hong-min;CHEN Jun-chang;LIU Yi-heng;WANG Kun-zheng. Experimental study of the effects of puerarin on biological characters of osteoblasts in vitro[J]. China Journal of Chinese Materia Medica, 2005, 30(24): 1947-1949 |
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| Authors: | ZANG Hong-min CHEN Jun-chang LIU Yi-heng WANG Kun-zheng |
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| Affiliation: | Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. zhm197@163.com |
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| Abstract: | Objective: To observe the effect of puerarin on cell proliferation,differentiation,maturation and mineralization in cultured rat osteoblasts.Method: Osteoblasts from craniums of newly born SD rats were cultured in vitro.MTT,PNPP and ARS were used to observe the proliferation,activity of ALP and the number of mineral node of cultured osteoblasts in vitro.Result: It was found that puerarin had the effect on stimulating cell proliferation,activity of ALP and the number of mineral node of cultured osteoblasts(P<0.01 or P<0.05).Conclusion: Puerarin can promote proliferation,differentiation,maturation and mineralization of the osteoblasts in vitro. |
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| Keywords: | puerarin cell culture osteoblasts osteoporosis |
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