大鼠脾虚证与血清甲状腺激素及下丘脑、胸腺细胞核T3受体关系

目的：探讨脾虚证与血清甲状腺激素及下丘脑、胸腺细胞核T3受体的关系。方法：成年SD大鼠32只了随机分为4组（每组8只）：A组（正常组）、B组（脾虚组）、C组（治疗组）、D组（自复组）。采用放射配基分析法，测定各组大鼠血清T3、T4、FT3、FT4、 γ－T3 及下丘脑、胸腺细胞核T3受体的含量。结果：B、D组大鼠血清T3、T4、FT3、FT4含量均较A、C组低，有显差异（P＜0.05或P＜0.01）；各且间血清γ－T3 含量无显性差异；B、D组下丘脑、胸腺细胞T3受体含量较A、C组低，有显差异（P＜0.01）。结论：血清甲状腺激素及下丘脑、胸腺细胞核T3受体的水平降低，可导致甲状腺激素生物效应低下，经由神经内分泌免疫调节环路，削弱免疫功能，这可能是脾虚证的一种重要病机。

RELATIONSHIP BETWEEN SPLEEN-ASTHENIA SYNDROME AND SERUM THYROXIN,T3 RECEPTORS OF HYPOTHALAMUS CELLS & THYMUS T LYMPHOCYTE NUCLEI IN RATS

Objective：To study the relationship between Spleen-asthenia syndrome and serum thyroxin,T3 receptors of hypothalamus cells & thymus T lymphocyte nuclei in rats.Method:Rhubarb decoction was used to develop spleen-asthenia syndrome in SD adult rat.32 rats were averagely divided into four groups at random：Group A(control group),group B(spleen-asthenia group),group C(group treated with Sijunzi decoction),group D(spontaneous rehabilitation group).The radioimmunoassay was applied to measure serum thyroxin levels and the contents of T3 receptors of hypothalamus cells and thymus T lymphocyte nuclei of every rat.Result:The serum levels of T3，T4，FT3，FT4，the contents of T3 receptors of hypothalamus cells and thymus T lymphocyte nuclei in group B,D were lower than those in group A,C.The difference between them was significant.Serum level of γ-T3 in four groups had no significant difference.Conclusion:The decline of T3,T4,FT3,FT4 serum levels,and the contents of nuclear T3 receptors in hypothalamus cells & thymus T lymphocytes can influence the activity of thyroxin,then the immunity function will be impaired via neuroendocrinimmunology net(NEI net),this may be a kind of important pathogensis of spleen-asthenia syndrome.