| 联氨基姜黄素脂质体纳米颗粒对MDA-MB-231细胞增殖、凋亡、侵袭及迁移的影响 |
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| 引用本文： | 田文霞,张曦文,王晓飞,唐浩,党微旗,罗奕,孔嵌蚺,陈婷梅.联氨基姜黄素脂质体纳米颗粒对MDA-MB-231细胞增殖、凋亡、侵袭及迁移的影响[J].中草药,2011,42(9):1773-1778. |
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| 作者姓名： | 田文霞 张曦文 王晓飞 唐浩 党微旗 罗奕 孔嵌蚺 陈婷梅 |
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| 作者单位： | 重庆医科大学医学检验系临床检验诊断学教育部重点实验室,重庆,400016 |
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| 基金项目： | 国家自然科学基金，重庆市科委自然科学基金 |
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| 摘 要： | 目的探讨联氨基姜黄素(hydrazinocurcumin,HC)脂质体纳米颗粒(NPs)对人乳腺癌细胞株MDA-MB-231增殖、凋亡、侵袭及迁移的影响。方法通过薄膜分散-超声法制备联氨基姜黄素脂质体纳米颗粒(HC-NPs)。用HC-NPs处理细胞后,MTT法检测细胞增殖,刘氏染色法检测细胞形态学改变,流式细胞术检测细胞周期与凋亡,Transwell检测细胞的侵袭和迁移,Western blotting检测与细胞周期、凋亡、侵袭及迁移相关蛋白分子的变化。结果 HC-NPs可抑制MDA-MB-231细胞的增殖,使细胞形态变圆,诱导细胞发生G2/M期阻滞,使细胞凋亡率明显增加(P<0.01),抑制细胞的侵袭和迁移。HC-NPs可下调磷酸化-信号转导和转录激活因子(p-STAT3)以及下游分子Cyclin D1、Survivin、Bcl-2及MMP-9的表达,上调Bax的表达。结论 HC-NPs可能通过抑制STAT3的激活,抑制细胞增殖、侵袭和迁移,促进细胞凋亡。
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| 关 键 词： | 联氨基姜黄素 脂质体纳米颗粒 人乳腺癌 MDA-MB-231 凋亡 侵袭 迁移 |
| 收稿时间： | 2011/4/23 0:00:00 |
Effects of liposome nanoparticles of hydrazinocurcumin on cell proliferation,apoptosis,invasion,and migration in MDA-MB-231 cells |
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| TIAN Wen-xi,ZHANG Xi-wen,WANG Xiao-fei,TANG Hao,DANG Wei-qi,LUO Yi,KONG Qian-ran and CHEN Ting-mei.Effects of liposome nanoparticles of hydrazinocurcumin on cell proliferation,apoptosis,invasion,and migration in MDA-MB-231 cells[J].Chinese Traditional and Herbal Drugs,2011,42(9):1773-1778. |
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| Authors: | TIAN Wen-xi ZHANG Xi-wen WANG Xiao-fei TANG Hao DANG Wei-qi LUO Yi KONG Qian-ran and CHEN Ting-mei |
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| Institution: | TIAN Wen-xia,ZHANG Xi-wen,WANG Xiao-fei,TANG Hao,DANG Wei-qi,LUO Yi,KONG Qian-ran,CHEN Ting-mei Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,Chin |
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| Abstract: | Objective To investigate the effects of liposome nanoparticles of hydrazinocurcumin(HC-NPs) on cells proliferation,apoptosis,invasion,and migration in human breast cancer MDA-MB-231 cells.Methods Liposome HC-NPs were prepared by film-sonication method and then were used to treat human breast cancer MDA-MB-231 cells.The treated cell survival was analyzed by MTT;Morphological changes were detected by Liu staining;The cell cycle and apoptosis were investigated by FCM assay;Then invasion and migration of the ce... |
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| Keywords: | hydrazinocurcumin(HC) liposome nanoparticles human breast cancer MDA-MB-231 apoptosis invasion migration |
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