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| 大孔吸附树脂分离纯化单叶铁线莲总皂苷的研究 | |
| 引用本文： | 孙晓博,张凯,李兰林,向大雄.大孔吸附树脂分离纯化单叶铁线莲总皂苷的研究[J].中草药,2011,42(10):1977-1981. |
| 作者姓名： | 孙晓博 张凯 李兰林 向大雄 |
| 作者单位： | 1. 中南大学湘雅二医院湖南省中药制剂新技术重点研究室,湖南长沙410011;益阳市中心医院,湖南益阳413000 2. 中南大学湘雅二医院湖南省中药制剂新技术重点研究室,湖南长沙410011;中南大学药学院,湖南长沙410013 3. 中南大学湘雅二医院湖南省中药制剂新技术重点研究室,湖南长沙,410011 |
| 基金项目： | 湖南省中医药科研计划项目(2010042) |
| 摘 要： | 目的研究大孔吸附树脂分离纯化单叶铁线莲总皂苷的工艺条件,为单叶铁线莲总皂苷的工业化生产提供实验依据。方法采用比色法测定总皂苷;比较了5种大孔吸附树脂的静态饱和吸附量、静态解吸率,筛选出适宜的树脂;对最佳树脂进行动态吸附、解吸试验,确定最佳的吸附洗脱工艺。结果 D-101大孔树脂对单叶铁线莲总皂苷有较好的吸附分离性能。最佳工艺条件为:上样液质量浓度为生药0.2 g/mL,pH值为5,上样体积流量为1 BV/h;4 BV蒸馏水洗脱杂质;4 BV 70%乙醇洗脱总皂苷,洗脱体积流量2 BV/h。按此工艺条件生产的总皂苷质量分数达到71.53%,收率86.02%,精制度达238.81%。结论 D-101大孔吸附树脂适合富集纯化单叶铁线莲中总皂苷。 |
| 关 键 词： | 单叶铁线莲 总皂苷 大孔吸附树脂 分离纯化 比色法 |
| 收稿时间： | 2010/12/11 0:00:00 |
Separation and purification of total saponins from Clematis henryi by macroporous resins |
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| SUN Xiao-bo,ZHANG Kai,LI Lan-lin and XIANG Da-xiong.Separation and purification of total saponins from Clematis henryi by macroporous resins[J].Chinese Traditional and Herbal Drugs,2011,42(10):1977-1981. | |
| Authors: | SUN Xiao-bo ZHANG Kai LI Lan-lin and XIANG Da-xiong |
| Institution: | SUN Xiao-bo1,3,ZHANG Kai1,2,LI Lan-lin1,XIANG Da-xiong1,2 1.Key Laboratory of Chinese Materia Medica Preparation of Hunan Province,The Second Xiangya Hospital of Central South University,Changsha 410011,China 2.School of Pharmceutical Sciences,Central South University,Changsha 410013,China 3.Yiyang Central Hospital,Yiyang 413000,China |
| Abstract: | Objective To study the technological parameters of enriching and purifying total saponins from Clematis henryi by macroporous resins.Methods The content of saponins was determined by colorimetry;The optimum macroporous resins were selected by comparing static adsorptive capabilities and desorption ratio of five kinds of resins;The dynamic adsorption and desorption of D-101 resin were researched to obtain the optimum parameters.Results The D-101 resin showed the best adsorptive and desorptive capabilities;Th... |
| Keywords: | Clematis henryi Oliv total saponins macroporous resins separation and purification colorimetry |
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