脐血间充质干细胞移植对大鼠局灶性脑缺血的影响

目的　从人脐血中分离纯化间充质干细胞(MSC) ,观察其移植对大鼠大脑中动脉栓塞后神经功能恢复的影响及细胞的存活、迁移向神经细胞分化的情况。方法　雄性SD大鼠45只,用线栓法建立大鼠大脑中动脉栓塞(MCAO)模型,大鼠随机分为3组:MSC移植组、单核细胞组和生理盐水组。移植后1、7、14、2 1、2 8d采用改良神经功能损害评分(mNSS)观察大鼠神经功能恢复情况,应用免疫组织化学和免疫荧光双标记技术检测5溴 2脱氧尿核苷(BrdU )标记的MSC细胞的存活、迁移及其胶质纤维酸性蛋白(GFAP)和神经元特异性核蛋白(NeuN)的表达。结果　人脐血MSC细胞移植可显著提高大鼠局灶性脑缺血后神经功能的恢复(P <0 .0 5 )。移植的MSC细胞可在大鼠脑组织中存活,并向缺血区域迁移,11.67%MSC细胞表达GFAP ,3 .72 %MSC细胞表达NeuN。结论　人脐血中含有MSC细胞并可促进局灶性脑缺血大鼠的神经功能恢复,移植细胞可在大鼠脑缺血区域中存活、迁移并向星形胶质细胞或神经元分化

Effect of transplantation of mesenchymal stem cells from umbilical cord blood after cerebral ischemia in rats

Objective To observe the neurological functional recovery after transplanting mesenchymal stem cells (MSCs) dirived from human umbilical cord blood to rats with middle cerebral artery occlusion (MCAO),and detect the survival time,migration and neural differentiation of MSCs.Methods Forty-five adult male SD rats were randomly dividedinto 3 groups:(1) Transplantation of MSCs from human cord blood after MCAO;(2)Transplantation of mononuclear cells from adult human blood after MCAO;(3) Injection of equivalence saline after MCAO.Functional outcome measurements were performed accoring to the modified neurological severity score on the day 1,7,14,21 and 28 after transplantation.The survival,migration,the expression of glial fibriliary acidic protein (GFAP) and neuron marker-NeuN (neuronal nuclei) of BrdU-labeled MSCs were detected by using immunohistochemical and immunofluorescent staining.Results The transplantation of MSCs could significantly improve the rats' neurological functional recovery after MCAO ( P < 0.05). The engraft cells could survive and migrate in ischemic tissue.About 11.67% and 3.72% MSCs expressed GFAP and NeuN maker respectively.Conclusion Human umbilical cord blood contains MSC which can significantly improve neurological recovery after MCAO in rats.The engraft cells can survive and migrate in ischemic tissue.Parts of MSCs can differentiate into astrocytes or neurons.MSC from human cord blood may play a great role in the treatment of the central nerve system diseases.