互隔交链孢霉诱发姐妹染色单体互换及染色体畸变的研究

本文介绍了10株互隔交链孢霉提取物诱发人淋巴细胞姐妹染色单体互换(SCE)及染色体畸变。结果发现,当用不同浓度提取物加入培养基时,有8株所诱发的SCE增高,与对照组比较差异极为显著(P<0.01)、在8株阳性株中,有6株在加入低量提取物时即诱发SCE频率增高显示统计学差异、同时,在阳性株中有两株提取物所诱发的染色体畸变频率增高,与对照组比较亦有极显著性差异(P<0.01)。由此可见,多数互隔交链孢霉菌株(8/10)所产生的代谢产物或毒素,都含有致突变物,不同菌株具有不同强度的致突作用。另外,对应用人淋巴细胞的SCE技术检测致突变物的优点进行了讨论。

Study on sister chromatid exchanges and chromosome aberrations induced by Alternaria alternata

It is indicated that sister chromatid exchanges (SCE) and chromosome aberrations in human lymphocytes can be induced by the extracts of 10 strains of Alternaria alternata. We found that the SCE frequencies increased in 8 strains when their extracts of different concentrations were added to the cul tures. The difference was quite significant, as compared with the control. In these 8 positive strains, there were 6 in which the increase of SCE requencies was induced and showed stastistical difference when extract of low dose was added to them. Therefore, we may say that the metabolic products or poisons produced by most strains of Alternaria alternata have mutagens, and the intensity of the effect of the mutagens varies with different strains. The advantage to detect the mutagens by using the SCE technique for human lymphocytes was discussed.