Physical map of the COB region in mitochondrial DNA of Saccharomyces cerevisiae

Summary A physical map of the COB region in mtDNA of yeast has been established. This region harbours a split gene coding for apocytochrome b. It includes restriction sites of seven endonucleases (EcoRI, HindII, HindIII, HaeIII, HpaII, AluI and BamHI). Various mtDNA sequences of this region, retained in a series of genetically characterized rho^– clones have been allocated to this map. The combination of this physical map with a genetic map of the rho^– clones revealed that 1) cob^– mutational sites spread over 8,400 bp, 2) mutations in sequences coding for apocytochrome b map in five distinct segments which are separated by intervening sequences with minimum lengths from 350 to 1,900 bp.Abbreviations mtDNA mitochondrial DNA - b bases - bp basepairs