| 不同浓度异氟醚对小鼠肺表面活性蛋白A表达的影响 |
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| 引用本文： | 韩芳,王丽霞,单智焱,王振东,刘春佳,雷蕾.不同浓度异氟醚对小鼠肺表面活性蛋白A表达的影响[J].解剖科学进展,2012,18(5):416-418. |
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| 作者姓名： | 韩芳 王丽霞 单智焱 王振东 刘春佳 雷蕾 |
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| 作者单位： | 1. 哈尔滨医科大学组织学与胚胎学教研室,黑龙江哈尔滨150081;哈尔滨市第五人民医院,黑龙江哈尔滨150040
2. 哈尔滨医科大学组织学与胚胎学教研室,黑龙江哈尔滨,150081 |
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| 基金项目： | 黑龙江省留学归国人员科学技术专项资金资助项目 |
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| 摘 要： | 目的 探讨异氟醚对小鼠肺表面活性蛋白A表达的影响。方法 将雄性昆明小鼠随机分为5组,吸入空气组、吸入1.0MAC异氟醚5min组、吸入3.0MAC异氟醚5min组、吸入1.0MAC异氟醚2h组、吸入3.0MAC异氟醚2h组;RT-PCR方法检测小鼠肺内SP-A mRNA的表达;免疫组化方法检测小鼠肺内SP-A蛋白的表达。结果 吸入异氟醚5min对小鼠肺内SP-A表达无影响,吸入异氟醚2h能降低小鼠肺内SP-A表达,而且随吸入浓度升高,SP-A表达呈负相关。结论 吸入高浓度异氟醚长时间能够降低小鼠肺内SP-A的表达。
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| 关 键 词： | 异氟醚 肺表面活性蛋白A 小鼠 |
Effect of Isoflurane with different concentrations on the expression of surfactant protein A in mouse lung |
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| HAN Fang
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WANG Li-xia
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SHAN Zhi-yan
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WANG Zhen-dong
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LIU Chun-jia
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LEI Lei.Effect of Isoflurane with different concentrations on the expression of surfactant protein A in mouse lung[J].Progress of Anatomical Sciences,2012,18(5):416-418. |
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| Authors: | HAN Fang WANG Li-xia SHAN Zhi-yan WANG Zhen-dong LIU Chun-jia LEI Lei |
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| Institution: | 1. Departerment of Histology and Embryology, Harbin Medical University, Harbin 150081; 2. The Fifth Harbin People Hospital, Harbin 50040, China ) |
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| Abstract: | Objective To study the effect of isoflurane on the expression of surfactant protein A in mouse lung. Methods Male Kunming mice were randomly divided into 5 groups： air group, 1.0MAC isoflurane for 5min group, 3.0MAC isoflurane for 5min group, 1.0MAC isoflurane for 2h group and 3.0MAC isoflurane for 2h group. The expression of SP-A mRNA and protein in mice lung was examined by RT-PCR and by immunohistoehemistry respectively. Results Inhalation of isoflurane for 5min had no effect on SP-A expression in mouse lung, inhalation of isoflurane for 2h reduced the SP-A expression level in mouse lung dosedependently. Conclusion Inhalation of isoflurane for long time at high concentration inhibits SP-A expression in mouse lung. |
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| Keywords: | Isoflurane surfactant protein A mouse |
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