血管紧张素原基因M235T多态性与冠心病的相关性研究

目的研究血管紧张素原基因M235T多态性与冠心病的关系。方法应用多聚酶链反应结合限制性内切酶法(PCRRFLP)对100名经冠脉造影确诊为冠心病(CHD)的患者(其中47名有心肌梗死病史)和52名冠造结果正常及18名门诊常规体检且无冠心病史者进行病例-对照研究,并进行多因素分析。结果病例组与对照组基因型分布及等位基因频率均有显著差异(P<0.01),CHD组TT基因型和T等位基因频率显著高于对照组(分别为0.67,0.79vs0.44,0.63),在心肌梗死患者中更明显(分别为0.74,0.85vs0.44,0.63)。性别对冠心病的发病也有影响,男性患冠心病发病的危险性是女性的1.899倍。结论AGT基因变异与CHD发病具有相关性。T等位基因是CHD的危险因素,可增加冠心病发病的2.059倍。性别也可作为冠心病的危险因素。

Relationship between angiotensinogen gene M235T polymorphism and coronary heart disease

Objective To study the relationship between angiotensinogen gene M235T polymorphism and coronary heart disease(CHD). Methods Polymerase chain reaction combined with restriction enzyme digestion (PCR-RFLP) was used to exploe the relationship between 100 CHD patients who were identified by coronary angiography(including 47 myocardial infarction individuals) and 70 subjects without CHD as control group(including 52 persons with normal coronary angiograph and 18 without clinical evidence of CHD). The multivariate analysis was available. Results There was obviously difference in the distributuion of genetype and T allele frequency between patients and controls(P<0.01).The frequency of TT genetype and T allele was significantly higher in patients than in controls(0.67,0.79 vs 0.44,0.63 respectively),it was more obviously in MI patients(0.74,0.85 vs 0.44,0.63 respectively).The incidence of CHD was also associated with sex,which was about 1.899 times in men vs in women. Conclusion There is a correlation between gene variants of the angiotensinogen and incidence of CHD.T allele is a risk factor,and increase the risk of CHD by 2.059.Sex may be a risk factor of CHD.