| 9-顺-维甲酸对非小细胞肺癌CyclinD1、Cdk4表达的影响 |
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| 引用本文： | 任桂杰,刘志方,丁磊,胡国强,胡晓燕,于雪艳. 9-顺-维甲酸对非小细胞肺癌CyclinD1、Cdk4表达的影响[J]. 山东大学学报(医学版), 2003, 41(3) |
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| 作者姓名： | 任桂杰 刘志方 丁磊 胡国强 胡晓燕 于雪艳 |
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| 作者单位： | 山东大学医学院生化与分子生物学研究所;山东大学齐鲁医院胸外科 |
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| 基金项目： | 国家自然科学基金资助项目(39770730) |
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| 摘 要： | 目的:研究9-顺-维甲酸对非小细胞肺癌A549、PG、SPC-A1的细胞周期蛋白D1(cyclinD1)、细胞周期蛋白依赖性蛋白激酶4(Cdk4)表达的影响,探讨其抑制非小细胞肺癌生长与影响CyclinD1、Cdk4表达的相关性。方法:采用半定量逆转录PCR方法,分析细胞周期因子CyclinD1、Cdk4表达的变化。结果:9-顺-维甲酸既可以降低PG、SPC-A1的CyclinD1表达(P<0.01),又可以降低PG、A549的Cdk4表达(P<0.01)。结论:9-顺-维甲酸对非小细胞肺癌生长的抑制作用,与其调控细胞周期因子CyclinD1、Cdk4的表达密切相关。
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| 关 键 词： | 维甲酸 癌 非小细胞肺 细胞周期蛋白D1 细胞周期蛋白依赖性蛋白激酶4 |
Effects of 9-cis-retinoic acid on expression of CyclinD1 and Cdk4 in non-small cell lung cancer |
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| REN Gui-jie,LIU Zhi-fang,DING Lei,et al. Effects of 9-cis-retinoic acid on expression of CyclinD1 and Cdk4 in non-small cell lung cancer[J]. Journal of Shandong University:Health Sciences, 2003, 41(3) |
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| Authors: | REN Gui-jie LIU Zhi-fang DING Lei et al |
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| Abstract: | Objective:To study the effects of9-cis-retinoic acid(9-cis-RA)on the expression of CyclinD1and Cdk4in non-small-cell lung cancer PG,A 549 and SPC-A1,and to investigate the correla-tion between inhibition of cell growth by9-cis-RA and interference with the expression of CyclinD1and Cdk4in non-small-cell lung cancers.Method s:RT-PCR was used to analyze the changes in the expression of CyclinD1and Cdk4of PG,A 549 and SPC-A1.Results:9-cis-RA can not only decrease the transcription of CyclinD1of PG and SPC-A1(P<0.01),but also decrease the transcription of Cdk4of PG and A 549 (P<0.01).Conclu sion:The growth-inhibitory effect of9-cis-RA on non-small-cell lung cancers was tightly correlated with its mediation of the transcription of CyclinD1and Cdk4. |
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| Keywords: | Retinoic acid Non-small-cell lung cancers CyclinD1 Cdk4 |
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