中国四川地区涎腺淋巴上皮癌的EB病毒原位杂交研究

目的探讨在中国四川地区EB病毒感染和涎腺淋巴上皮癌发病的关系。方法采用一个针对EB病毒编码的小分子RNA(EBER-1)的寡核苷酸探针对16例涎腺淋巴上皮癌(LEC)、4例良性淋巴上皮病损(BLEL)、4例非特异性慢性涎腺炎石蜡包埋组织进行了原位杂交检测。结果本组病例中16例LEC的癌细胞核均见EBER-1强阳性信号，4例BLEL、4例非特异性慢性涎腺炎及2例瘤旁正常涎腺组织均为阴性。结论本结果提示EB病毒与中国四川地区涎腺淋巴上皮癌发病有密切关系。

Epstein-barr Virus Infection in Lymphoepithelial Carcinoma of Salivary Glands in Sichuan Chinese

Objective To determine the prevalence of Epstein Barr virus(EBV) infection in lymphoepithelial carcinoma (LEC) of salivary glands in Sichuan Chinese and discuss the role of EBV infection in pathogenesis of LEC. Methods Paraffin sections of 16 cases of LEC and 4 cases of benign lymphoepithelial lesion(BLEL)of salivary glands, 4 cases of non specific chronic sialadenitis were examined by in situ hybridization for EBV encoded small RNA 1(EBER 1) using 30 base synthesized oligonucleotide probe. Results Strong signals for EBER 1 were obtained in most of tumor cell nuclei in all the specimens of LEC(16/16); in contrast, there were no significant signals observed in the examination of 4 cases of BLEL and 4 cases of non specific chronic sialadenitis. Conclusion The results indicate that EBV plays an important role in the pathogenesis of LEC of salivary glands in Sichuan Chinese.