电针对慢性强迫游泳应激模型大鼠抑郁样行为及血清5-HT的影响

目的：在慢性强迫游泳应激模型上,观察电针对大鼠行为及血清五羟色胺（5-HT）含量的影响。方法：将雄性SD大鼠随机分为正常对照组、模型组、电针组和氟西汀组,每组10只。采用慢性强迫游泳应激方法制备抑郁大鼠模型。通过体质量、旷场实验、糖水实验对大鼠进行行为学评价,用ELISA方法检测大鼠血清中的5-HT含量。结果：与正常组比较,模型组大鼠体质量、旷场实验的水平运动和垂直运动次数、糖水摄入量均明显下降（P〈0.05）,血清5-HT含量降低（P〈0.05）。与模型组比较,电针组和氟西汀组大鼠体质量、旷场实验中的平行活动和垂直运动次数、糖水摄入量均明显增加（P〈0.05）,血清中的5-HT含量明显增加（P〈0.05）,电针组与氟西汀组之间比较,无显著性差异（P〉0.05）。结论：电针可改善慢性强迫游泳应激导致的大鼠抑郁样行为异常,明显增加血清中5-HT含量,具有抗抑郁作用。

Effects of Electroacupuncture on Depressive -like Behavior and Serum 5 -HT in the Chronic Forced Swim Stress Model Rats

Objective ： To observe the effects of electroacupuncture （EA） on depressive - like behavior and serum 5 -hydroxytryptamine （5 -HT） of the chronic forced swim stress model rats. Methods： 40 male SD rats were evenly randomized into normal control, model, fluoxetine and EA groups. The chronic stress rat model was established by chronic forced swim. The behaviors were evaluated by body weight, open field test and su- crose intake test, the serum 5 - HT was detected by ELISA. Results ： Compared with the control group, the body weight, crossings and rearings in the open field test, the sucrose intake, and the serum 5 - HT of model group were decreased significantly （P 〈 0.05）. Compared with the model group, the body weight, crossings and rearings in the open field test, the sucrose intake, and the serum 5 - HT of fluoxetine group and EA group were all increased significantly （ P 〈 0.05 ）. But there was no significant difference between EA group and flu- oxetine group （ P 〉 0.05 ）. Conclusion ： Electroacupuncture can improve the model rats＇ depressive - like behaviors which are caused by chronic forced swim stress, and may increase the serum 5 - HT of the model rats. Hereby, electroacupuncture may have anti -depressive effects.