| 细菌脂多糖诱导人胃上皮癌细胞株BGC-823环氧化酶-2的合成 |
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| 引用本文： | 李莲 冯桂香 任永生 王伟 熊顺华. 细菌脂多糖诱导人胃上皮癌细胞株BGC-823环氧化酶-2的合成[J]. 郧阳医学院学报, 2004, 23(2): 85-87 |
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| 作者姓名： | 李莲 冯桂香 任永生 王伟 熊顺华 |
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| 作者单位： | 郧阳医学院生理学教研室 湖北十堰442000(李莲，冯桂香，任永生，王伟)，郧阳医学院生理学教研室 湖北十堰442000(熊顺华) |
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| 基金项目： | 湖北省教育厅科研基金资助项目(鄂教科 [2 0 0 3]5号 ) |
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| 摘 要： | 目的 :研究细菌脂多糖 (lipopolysaccharide,LPS)对人胃上皮细胞环氧化酶 1和环氧化酶 2合成的影响。方法 :实验选用LPS(E .Coli 0 111∶B4 )和人胃上皮癌细胞株BGC - 82 3。环氧化酶的合成用免疫蛋白印迹法(WesternBlotting)测定。 结果 :LPS对BGC 82 3细胞环氧化酶 2的合成有明显的诱导作用。BGC - 82 3细胞环氧化酶 - 2的含量在LPS作用 1h后明显增加 ,并持续至少 6h。结论 :E .Coli的LPS可诱导BGC - 82 3细胞COX- 2的合成。
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| 关 键 词： | 脂多糖 胃肿瘤 上皮细胞 环氧化酶 |
| 文章编号： | 1006-9674(2004)02-0085-03 |
| 修稿时间： | 2004-03-02 |
Lipopolysaccharide induces the synthesis of cyclooxygenase-2 in human gastric carcinoma epithelial cell line BGC-823 |
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| LI Lian,FENG Gui-xiang,REN Yong-sheng,et al. Lipopolysaccharide induces the synthesis of cyclooxygenase-2 in human gastric carcinoma epithelial cell line BGC-823[J]. Journal of Yunyang Medical College, 2004, 23(2): 85-87 |
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| Authors: | LI Lian FENG Gui-xiang REN Yong-sheng et al |
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| Abstract: | Objective To investigate the effect of lipopolysaccharide (LPS) on the synthenase of cyclooxygenase-1 and cyclooxygenase-2 in human gastric epithelial cell line, BGC-823. Methods LPS (E. Coli 0111:B4) and BGC-823 were used in our study. The synthesis of cyclooxygenase was examined by Western blot. Results The results of Western blot showed that the content of cyclooxygenase-2 increased obviously after the cells incubated with lipopoly saccharide a hour and it could last for six hours at least, the quantity of COX-1 did not change during this period. Conclusions E. Coli LPS would be effective stimuli of the synthesis of COX-2 in gastric carcinoma epithelial cell line. |
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| Keywords: | lipopolysaccharide stomach neoplasms epithelial cells cyclooxygenase |
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