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Immunoperoxidase assay for the detection of specific IgG antibodies to Hantaan virus |
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| Authors: | G van der Groen G Beelaert |
| Affiliation: | Department of Virology, Institute of Tropical Medicine, Nalionalestraat 155, B-2000 Antwerpen, Belgium |
| Abstract: | A technique, using indirect immunoperoxidase antibody (IPA), was developed for the detection of IgG antibody to Hantaan virus. The same protein A-peroxidase conjugate was used with mouse, rat and human sera. The IPA technique employs glass slides with air-dried gamma-ray-inactivated and acetone-fixed Hantaan-infected Vero-E6 cells. Antibody titers detected by IPA was comparable to those detected by the indirect fluorescent antibody technique. |
| Keywords: | Hantaan antibodies IgG protein A-peroxidase indirect immunoperoxidase indirect immunofluorescent antibody test Hantaan-infected Vero-E6 cells |
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