Noradrenergic feeding system in monkey hypothalamus is altered by localized perfusion of glucose,insulin, 2-DG and eating

Hypothalamic sites in the monkey were labelled by micro-injections of ³H-NE and successive push-pull perfusions were carried out at a rate of 25 μl/min. When the monkey was fed, ³H-NE within the perifornical region increased. When 2-deoxy-D- glucose (2-DG) was added to the perfusate, ³H-NE release was also enhanced, whereas insulin perfused at the same rate caused a delayed increase in catecholamine levels as reflected by increased radioactivity. Glucose supressed the release of ³H-NE, suggesting overall that the noradrenegic feeding system in the hypothalamus of the monkey is modulated by the regional level of glucose as well as the local concentration of insulin.