乙酰葛根素对 Aβ25-35诱导BV-2小胶质细胞活性的影响

目的：研究乙酰葛根素对β淀粉样蛋白（ Aβ25-35）诱导的BV-2小胶质细胞活性的影响。方法体外培养BV-2小胶质细胞，用Aβ25-35诱导小胶质细胞建立AD细胞模型，加入不同浓度的乙酰葛根素，显微镜下观察细胞的形态变化；MTT法检测细胞的存活率；ELISA法检测细胞中IL-1β，TNF-α的含量。结果 Aβ诱导后小胶质细胞由分枝状的静息状态转变为阿米巴样的激活状态，而乙酰葛根素可以减轻细胞发生的变化，细胞状态介于空白组与模型组之间；MTT显示乙酰葛根素不会对小胶质细胞的存活率产生影响；ELISA结果显示，与空白组相比，模型组的IL-1β，TNF-α表达水平明显升高，而乙酰葛根素能够降低Aβ诱导IL-1β，TNF-α的表达水平。结论乙酰葛根素能够抑制Aβ25-35诱导的小胶质细胞激活减轻炎性因子的分泌，对阿尔茨海默病具有保护作用。

Effect of Acetylpuerarin on Aβ25-35-induced Activation of BV-2 Microglia

Objective To investigate the effect of Acetylpuerarin on Aβ25-35-induced activation of BV-2 microglia cells.Methods BV-2 microglia cells were cultured in vitro and stimulated with Aβ25-35 to establish the AD model cells .Then the cells were treated with dif-ferent concentration of Acetylpuerarin .The inverted phase contrast microscope was applied to observe the morphological changes of microglia . MTT assay was used to detect the viability of BV-2 cells.ELISA method was used to evaluate the concentration of IL-1βand TNF-αin BV-2 cells.Results Viability of BV-2 cells is not decreased after Acetylpuerarin simulation ,indicating Acetylpuerarin has no toxicity to BV-2 cells.compared with the control group ,the concentration of IL-1βand TNF-αin Aβ25-35 group were significantly increased ,and Acetylpuerarin could down-regulate the expression of IL-1βand TNF-α.Conclusion Acetylpuerarin can inhibit excessive activation of microglia induced by Aβ25-35 and decrease the inflammatory secretion ,it has protective effect on Alzheimer＇s disease.