CO2对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究

目的：探讨腹腔镜二氧化碳（CO2）气腹对宫颈癌细胞体外生长的影响。方法：体外培养人宫颈癌细胞株Caski，以14mmHg气压的CO2处理2h，用四唑盐比色试验（MTT）、流式细胞术（FCM）和免疫细胞化学法检测Caski细胞外生长情况，细胞周期变化及周期相关蛋白CDK4和Cyclin D1的表达水平。结果：CO2处理后Caski细胞增殖加快，细胞周期中处于增殖期的细胞比例增高，周期相关蛋白CDK4和CyclinD1的表达显著增加。结论：CO2可以促进Caski细胞的增殖。

Effects of carbon dioxide on the proliferation and CDK4 and Cyclin D1 expressions of human cervical carcinoma cell line CASKI

Objective:To study the effects of celioscope carbon dioxide pneumoperitoneum on the growth of cervical carcinoma cell line in vitro. Methods:Cervical carcinoma cells CASKI were cultured in vitro,and then treated with 14mmHg,100% carbon dioxide for 2h. The proliferation,cell cycle and expressions of CDK4 and Cyclin D1 were examined by MTT, FCM and immunocytochemistry respectively. Results:After the treatment of carbon dioxide, the proliferation of cervical carcinoma cells CASKI was significantly increased at the 3th day, the proportion of proliferative phase in the cell cycle was significantly increased, and the expressions of CDK4 and Cyclin D1 were also significantly increased. Conclusion:carbon dioxide may promote the proliferation of cervical carcinoma cell line.