小鼠无精子症动物模型的构建

目的:建立一种稳定的小鼠无精子症动物模型。方法:60只清洁级C5BL/6小鼠,分为化疗组(1次性腹腔注射白消安10mg/kg体重、环磷酰胺120mg/kg体重)、激素组(每日皮下注射苯甲酸雌二醇40mg/kg,连续注射15d),每组30只。注射后4、12、20周,观察小鼠睾丸生精小管结构的变化以及终止干预后生精功能恢复的情况。结果:化疗组化疗后第4周,睾丸生精小管内精子及精子细胞消失,20周时仍保持无生精状态;激素组连续注射苯甲酸雌二醇后第4周,生精小管内精子及精子细胞消失,但20周时部分生精小管内可以找到精子细胞和精子。化疗组小鼠睾丸重量明显低于激素组(P<0.05)。结论:经过化疗处理的小鼠无精子症模型稳定,雌二醇皮下注射的无精子症模型形成慢而不稳定。相对于雌激素注射方法,化疗是构建小鼠无精子症动物模型比较可靠的方法。

Establishment of an Animal Model of Azoospermia in Male Mice

OBJECTIVE: To establish a stable animal model of azoospermia in male mice. METHODS: Two groups of mice, with 30 in each, were intervened respectively by chemotherapy (intraperitoneal injection of Busulfan and Cyclophosphamide) and subcutaneous injection of Estradiol. At different times after the injection, the microscopic structures of the seminiferous tubules of both groups were observed and compared. The recovery of the germ cells in the seminiferous tubules was also evaluated after the termination of the intervention. RESULTS: A stable animal model was established by chemotherapeutic method with Busulfan and cyclophosphamide, while the model constructed by muscle injection of Estradiol was not stable. CONCLUSION: Compared with estrogen injection, chemotherapeutic intervention is a reliable method for constructing an animal model of azoospermia in male mice.