雄激素对大鼠腹叶前列腺GDNF mRNA表达的影响

目的 :探讨雄激素对大鼠腹叶前列腺中胶质细胞源性神经营养因子 (GDNF)mRNA表达的影响。　方法 :2 4只SD大鼠分为 3组 ,其中A组 (n =8)为假手术对照组 ,B组 (n =8)为去势组 ,C组 (n =8)为雄激素替代组 (去势后肌注十一酸睾酮 5 0mg/kg) ;术后 3d处死 ,通过半定量RT PCR检测GDNFmRNA在去势前后和雄激素替代组大鼠腹叶前列腺中的表达变化。　结果 :去势后大鼠前列腺的体积萎缩变小 ;雄激素替代组出现前列腺增生变大 ;对照组正常的大鼠前列腺有GDNFmRNA表达 ,去势组GDNFmRNA表达量减少 ,雄激素替代组GDNFmRNA表达量增加。与正常对照组比较 ,去势组的GDNFmRNA表达量显著减少 (P <0 .0 5 ) ,雄激素替代组的GDNFmRNA表达量显著增加(P <0 .0 5 )。　结论 :雄激素可增加GDNFmRNA表达 ,促进前列腺细胞生长。

Effects of Testosterone on GDNF mRNA Expression in Rat Ventral Prostate

Objective: To investigate the effects of testosterone on GDNF(glial cell derived neurotrophic factor) expression in rat ventral prostate. Methods: Twenty four male Sprague Dawley rats were randomized into 3 groups, group A( n =8, sham operation,uncastrated controls), group B ( n =8, castrated), group C ( n =8, castrated and given testosterone undecanoate (TU) 50 mg/kg by intramuscular injection). Ventral prostate tissues removed from adult male rats at 3 days after operation were analyzed for the expression of GDNF mRNA by semiquantitative RT PCR assay. Results: The ventral prostate tissues shrank remarkably in the castrated rats, and prostatic hyperplasia accurred in those that had received both castration and testosterone undecanoate. GDNF mRNA expressed in the normal rats' ventral prostates, and the prostatic expression of GDNF mRNA decreased significantly at 3 days after castration( P < 0.05 ), but increased significantly ( P < 0.05 ) in those that had received both castration and TU. Conclusions: The gene expression of glial cell derived neurotrophic factor in the ventral prostate of rats is dependent on testosterone, and GDNF is involved in the growth of rat ventral prostate.