Bcl2-siRNA提高人肺腺癌细胞A549/DDP对顺铂敏感性的研究

目的 探讨Bcl2-siRNA 对人肺腺癌顺铂耐药细胞株A549/DDP 对顺铂敏感性的影响.方法 在人肺腺癌顺铂耐药细胞株A549/DDP 细胞中转染bcl2-siRNA,48h 后加入不同浓度(0、3、6、9、12、15μg/ml) 新鲜配置的顺铂溶液,药物作用48h后,用CCK-8 法检测细胞生长抑制率.荧光实时定量PCR(Real time RT-PCR) 检测A549/DDP 细胞转染bcl2-siRNA 后Bcl-2mRNA 的变化,Western Blot 检测A549/DDP 细胞转染bcl2-siRNA 后Bcl-2 蛋白的变化.结果 在A549/DDP 细胞中转染bcl2-siRNA 后,细胞Bcl-2 mRNA 降低92.6%(P<0.05),Bcl-2 蛋白表达也明显降低,同时细胞对顺铂的敏感性增加57.8%(P<0.05).结论 Bcl2-siRNA 能降低A549/DDP 中Bcl-2 mRNA 及蛋白的表达,增加A549/DDP 对顺铂的敏感性.

Bcl2-siRNA increases sensitivity of human lung adenocarcinoma A549/DDP cells to cisplatin

Objective To study the effect of Bcl2-siRNA on sensitivity of cisplatin-resistant human lung adenocarcinoma A549/DDP cells to cisplatin.Methods Forty-eight hours after Bcl2-siRNA was transfected with cisplatin-resistant human lung adenocarcinoma A549/DDP cells,a fresh cisplatin solution was prepared by adding different concentrations of cisplatin(0μg/ml,3μg/ml,6μg/ml,9μg/ml,12μg/ml,15μg/ml) into A549/DDP cells to measure their growth-inhibition rate by CCK-8 assay.Expression of Bcl-2 mRNA and protein in A549/DDP cells-transfected Bcl2-siRNA was detected by Real time RT-PCR and Western blot,respectively.Results The expression of Bcl-2 mRNA and protein in A549/DDP cells-transfected Bcl2-siRNA decreased 92.6%(P<0.05) while the sensitivity of A549/DDP cells to cisplatin increased 57.8%(P<0.05).Conclusion Bcl2-siRNA can decrease the expression of Bcl-2 mRNA and protein in A549/DDP cells,and increase the sensitivity of A549/DDP cells to cisplatin.