| 秋鼠曲草花部分有效成分提取工艺优选 |
| |
| 引用本文： | 骆冲,孙慧,陈芳,赖家平.秋鼠曲草花部分有效成分提取工艺优选[J].中国实验方剂学杂志,2012,18(19):53-56. |
| |
| 作者姓名： | 骆冲 孙慧 陈芳 赖家平 |
| |
| 作者单位： | 1. 华南师范大学化学与环境学院,广州,510006
2. 广州大学环境科学与工程学院,广州,510006 |
| |
| 摘 要： | 目的:优选秋鼠曲草花中主要成分的提取工艺.方法:以槲皮素和芹菜素含量为综合评价指标,采用HPLC测定指标成分含量,采用单因素试验考察提取方式、料液比、提取次数及乙醇体积分数;选取提取次数、提取时间、料液比及乙醇体积分数为考察因素,正交设计试验优选提取工艺.结果:最佳提取工艺为超声提取,加入30倍量60％乙醇超声提取2次,每次40 min.结论:优选的提取工艺简单、稳定可行,可为秋鼠曲草花部分有效成分的定量分析提供可靠的提取方法.
|
| 关 键 词： | 秋鼠曲草花 槲皮素 芹菜素 正交试验 高效液相色谱法 |
| 收稿时间： | 2012/4/23 0:00:00 |
Optimization of Extraction Technology for Main Components from Flower of Gnaphalium hypoleucum |
| |
| LUO Chong,SUN Hui,CHEN Fang and LAI Jia-ping.Optimization of Extraction Technology for Main Components from Flower of Gnaphalium hypoleucum[J].China Journal of Experimental Traditional Medical Formulae,2012,18(19):53-56. |
| |
| Authors: | LUO Chong SUN Hui CHEN Fang and LAI Jia-ping |
| |
| Institution: | School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China;College of Environmental Science & Engineering, Guangzhou University, Guangzhou 510006, China;School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China;School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China |
| |
| Abstract: | Objective:To optimize extraction process of main components from flower of Gnaphalium hypoleucum. Method:With the contents of quercetin and apigenin as comprehensive evaluation index,which were determined by HPLC.Extraction method,ratio of solid-liquid,extraction times and the concentration of ethanol were investigated by single factor test;orthogonal design test was used to optimize extraction technology with extraction time,extraction times,ratio of solid-liquid and the concentration of ethanol. Result:Optimum extraction conditions were as follows:ultrasonic extracted 2 times with 30 times the amount of 60% ethanol,40 min per time. Conclusion:This optimized process was simple,stable and feasible.It could provide reliable extraction method for quantitative analysis of main components from flower of G. hypoleucum. |
| |
| Keywords: | flower of Gnaphalium hypoleucum quercetin apigenin orthogonal design HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
