Fas介导的细胞凋亡在乙型肝炎肝组织中分布状况初探

细胞凋亡可能是某些因素通过基因发动的一种细胞死亡过程。据认为,该过程主要通过Ｆａｓ抗原介导。为调查Ｆａｓ抗原表达与慢性乙型肝炎组织损伤的关系,本研究分别用免疫组化、原位末端标记法观察Ｆａｓ抗原表达及核ＤＮＡ断裂在慢性乙型肝炎肝组织中的分布状况。结果发现,Ｆａｓ抗原表达在活动性炎症区明显多于非炎症区,且主要位于汇管区及小叶内的炎症浸润区,５６例慢性乙型肝炎患者中,Ｆａｓ抗原阳性者４６例,阳性率８２％。直线相关回归分析提示,核ＤＮＡ断裂与Ｆａｓ表达明显相关。结果表明,Ｆａｓ介导的细胞凋亡在慢性乙型肝炎发病机制中具有重要作用

Primary study on Fas-mediated apoptosis in the liver tissue of patients with chronic hepatitis B]

Apoptosis may be the cause of cell death that activate gene due to certain factors. It has been suggested that apoptosis is mediated through Fas antigen. To evaluate the role of the Fas antigen and apoptosis in chronic hepatitis B (CHB), we observed the Fas expression and nuclear DNA fragement in CHB hepatic tissue using immunohistochemistry and TUNEL method respectively. The results showed that the Fas antigen expressing cells located mainly in inflammation infiltrating area but less in non-inflammation infiltrating area; the positive cells were found particularly among infiltrating lymphocytes at the advancing edges of "piecemeal necrosis" near the portal regions. The expression of Fas was detected mainly in the cytoplasm of hepatocytes. 46 samples from 56 CHB patients were Fas positive, the posive rate reached 82%. The Linear Correlation and Regression analysis suggested that there was a direct correlation between nuclear DNA fragement and Fas expression. These findings suggest that the apoptosis mediated through Fas antigen may play an important role in pathogenesis of CHB.