慢性阻塞性肺疾病患者血清和诱导痰中白细胞介素-32水平变化及意义

目的测定慢性阻塞性肺疾病（chronic obstructive pulmonary disease,COPD）急性加重期和稳定期患者血清和诱导痰中白细胞介素（interleukin,IL）-32水平,探讨IL-32在COPD发病中的作用。方法 60例COPD急性加重期患者（AECOPD组）,60例COPD稳定期患者（COPD稳定期组）及30名健康受试者（健康对照组）,分别进行痰细胞计数和分类,用ELISA法检测血清和诱导痰中IL-32和IL-8、肿瘤坏死因子-α水平,并分析IL-32水平与中性粒细胞、IL-8、肿瘤坏死因子-α及气流阻塞间的相关性。结果 AECOPD组血清及诱导痰中IL-32水平高于健康对照组和COPD稳定期组（P〈0.05）,COPD稳定期组高于健康对照组（P〈0.05）;血清及诱导痰中IL-32水平与IL-8、肿瘤坏死因子-α、中性粒细胞数及比例均呈正相关（P〈0.01）,与第1秒用力呼气容积占预计值百分比、第1秒用力呼气容积/用力肺活量和pa（O2）均呈负相关（P〈0.01）。结论 IL-32参与COPD的炎症过程,此过程与中性粒细胞、IL-8及肿瘤坏死因子-α等炎性因子有关。IL-32水平在一定程度上可反映COPD严重程度。

Changes and significance of interleukin-32 in the serum and induced sputum in chronic obstructive pulmonary disease

Objective To investigate the role of interleukin-32 in chronic obstructive pulmonary disease（COPD） by detecting the levels of interleukin-32 in the serum and induced sputum in patients with COPD.Methods Sixty patients with acute exacerbation of COPD,sixty patients with stable COPD and thirty healthy subjects were recruited.The concentrations of interleukin-32,interleukin-8 and tumor necrosis factor-alpha（TNF-α） in serum and induced sputum were measured with enzyme-linked immunosorbent assay（ELISA）.The correlations among interleukin-32,neutrophil,interleukin-8,TNF-α and lung functions were investigated.Results The levels of interleukin-32 in serum and sputum were significantly higher in acute exacerbation of COPD than those in healthy subjects and in stable COPD patients（P0.05）,and were higher in stable COPD patients than those in healthy subjects（P0.05）.The levels of interleukin-32 in serum and sputum were positively correlated with the levels of interleukin-8,TNF-α,and neutrophil count and percentage（P0.01）,and were negatively correlated with forced expiatory volume in one second（FEV1）/predicted values,FEV1/forced vital capacity and pa（O2）（P0.01）.Conclusion Interleukin-32 may be involved in the pathogenesis of airway inflammation in COPD.Interleukin-32 may be a noninvasive marker of severity of COPD.