早期低氧培养对体外受精-胚胎移植妊娠结局的影响

目的:探讨胚胎发育早期行5%O2培养对体外受精-胚胎移植妊娠结局的影响。方法:比较胚胎发育早期在5%O2(398例)和20%O2(221例)培养环境下的受精率、卵裂率、优质胚胎率、胚胎利用率、临床妊娠率等。结果:5%O2环境下的优质胚胎率及临床妊娠率均高于20%O2环境(P<0.01),但受精率、卵裂率、胚胎利用率等均无差异(P>0.05)。结论:对于第3天移植的胚胎,胚胎发育早期给予5%O2培养环境可提高胚胎发育潜能,有助于临床妊娠率的提高。

Effect of low oxygen concentration on outcome of in vitro fertilization - embryo transfer

Objective： To explore the effect of low oxygen concentration （5% O2 ） on the outcome of in vitro fertilization - em- bryo transfer （IVF- ET）. Methods： The fertilization rate, cleavage rate, good quality embryo rate, embryo utilization rate and clinical pregnancy rate in IVF -ET under different culture environments （5% O2 and 20% O2 ） were evaluated. Results： Higher good quality embryo rate and clinical pregnancy rate were found in the low oxygen concentration group （ P 〈 0.01 ）. There were no significant differences in the fertilization rate, cleavage rate and embryo utilization rate between the two groups （ P all 〉 0.05 ）. Conclusion ： Low oxygen concentrations maybe more suitable culture condition for early stage of human em- bryos during the first 3 days of in vitro development.