超声检查子宫内膜分型与孕酮受体表达的研究

目的:探讨超声波检测法评价子宫内膜分型的分子基础。方法:手术获取排卵日三线型(A型)和均匀型(C型)人窗口期子宫内膜,采用实时定量PCR(RT-PCR)、免疫组织化学方法研究孕酮受体A和B在子宫内膜中的基因表达水平和蛋白表达定位。结果:超声检测分型为A型和C型人窗口期子宫内膜中,RT-PCR结果显示孕酮受体B基因的mRNA表达有差异,孕酮受体A的mRNA表达差异不明显;免疫组织化学结果显示孕酮受体B蛋白质在子宫内膜间质细胞的表达有差异,孕酮受体A的表达差异不明显。结论:孕酮受体B在介导子宫内膜对孕酮的反应过程中起重要作用,可能是超声分型的分子基础。

Expression of PRA and PRB in ultrasound evaluation of endometrial receptivity

Objective： To explore the molecular basis of human endometrium of different patterns by ultrasound examination. Methods： The mRNA and protein expression levels of progesterone receptor A （PRA） and PRB were evaluated in different patterns of endometrium by real - time PCR and immunohistochemistry, respectively. Results ： There were differences in the mRNA and protein expression levels of PRB in the Pattern A and Pattern C endometria of the implantati3n window. No difference was found in PRA expression levels. Conclusion： PRB plays an important role in mediating the responsiveness of endometrium to progesterone during the implantation window in the endometria, which may be the molecular basis for the classification of ultrasonic patterns.