A型主动脉夹层组织Beclin1及微管相关蛋白1轻链3的表达及意义

目的 探讨自噬相关蛋白Beclin1及微管相关蛋白1轻链3(LC3)在A型主动脉夹层组织中的表达及意义。方法 选取因A型主动脉夹层(AD)而于我院行手术治疗患者术中切除的主动脉壁组织标本20例作为AD组,以尸检者的正常胸主动脉(NA)壁组织标本10例作为对照。采用免疫组化染色及蛋白质印迹技术检测血管组织中Beclin1及LC3蛋白的定位及表达差异。结果 A型主动脉夹层主动脉壁组织中Beclin1及LC3蛋白主要定位表达于血管平滑肌细胞,且含量高于正常对照组织(P<0.05)。结论 A型主动脉夹层主动脉壁中血管平滑肌细胞的自噬活性明显增强,可能与疾病的发生发展相关。

Expressions of Beclin1 and microtubule-associated protein 1 light chain 3(LC3) in type A aortic dissection tissues

Objective To explore the expressions of Beclin1 and microtubule-associated protein 1 light chain 3(LC3) in type A aortic dissection (AD) tissue and to discuss the relevance. Methods Twenty surgical AD specimens were used in this study, and 10 normal control specimens were obtained from autopsy. Immuno-histochemical staining and Western blotting analysis were performed to evaluate the locations and expressions of Beclin1 and LC3 proteins. Results It was found that Beclin1 and LC3 proteins were mainly located in the vascular smooth muscle cells of the AD tissues, and the expressions in the AD tissues were signicantly higher than those in the control group (P<0.05). Conclusion Autophagy is activated in the vascular smooth muscle cells of AD tissues, which may play an important role in the development and progression of thoracic aortic dissection.