| 竞争法酶联免疫吸附分析测定人类血浆载脂蛋白M监测方法的建立及应用 |
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| 引用本文： | 叶一舟,袁忠祥,施盛,焦国庆.竞争法酶联免疫吸附分析测定人类血浆载脂蛋白M监测方法的建立及应用[J].中国临床医学,2011,18(3):281-283. |
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| 作者姓名： | 叶一舟 袁忠祥 施盛 焦国庆 |
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| 作者单位： | 1. 上海交通大学附属上海市第一人民医院心血管外科,上海,200080
2. 南京医科大学附属无锡市人民医院心血管外科,江苏无锡,214000 |
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| 摘 要： | 目的：建立人类血浆载脂蛋白M（ApoM）竞争法酶联免疫吸附分析检测方法。方法：建立人类血浆ApoM蛋白竞争法酶联免疫吸附分析（ELISA）检测方法,与人类重组ApoM蛋白标准含量进行对照,并利用该方法对932份健康人群血浆ApoM蛋白进行检测。结果：竞争法ELISA能准确测定人类血浆ApoM的浓度,健康人群血浆ApoM的中位数值为20.58mg/L。结论：竞争法ELISA操作方便、准确,可用于人类血浆ApoM蛋白的检测,为人类血浆ApoM的基础和临床研究提供了科学基础。健康人群血浆ApoM蛋白浓度为20.58mg/L。
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| 关 键 词： | 载脂蛋白M 酶联免疫吸附测定 |
Establishment and Clinical Application of Human Plasma Apolipoprotein M Detecting by Competitive ELISA |
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| YE Yizhou,YUAN Zhongxiang,SHI Sheng,JIAO Guoqing.Establishment and Clinical Application of Human Plasma Apolipoprotein M Detecting by Competitive ELISA[J].Chinese Journal Of Clinical Medicine,2011,18(3):281-283. |
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| Authors: | YE Yizhou YUAN Zhongxiang SHI Sheng JIAO Guoqing |
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| Institution: | YE Yizhou YUAN Zhongxiang SHI Sheng JIAO Guoqing 1.Department of Cardiovascular Surgery,the First People's Hospital,Shanghai Jiaotong University,Shanghai 200080,China,2.Department of Cardiovascular Surgery,Wuxi People's Hospital,Nanjing Medical University,Wuxi 214000 |
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| Abstract: | Objective:To construct the method to detect the concentration of human plasma apolipoprotein M(ApoM) with competetive enzyme linked immunosorbent assay(ELISA),and to detect the clinical samples from Shanghai First People's Hospital.Methods:A competetive ELISA for the quantification of ApoM was constructed.Clinical normal healthy human plasma samples were detected.The reference values of normal healthy individuals were established.Results:The competetive ELISA was a simple and specific quantitative assay for... |
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| Keywords: | Apolipoprotein M Enzyme linked immunosorbent assay |
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