BD总皂苷对营养性肥胖大鼠纹状体细胞外液中5-HT、5-HIAA含量的影响

目的：研究BD总皂苷对营养性肥胖大鼠纹状体细胞外液中单胺类神经递质含量的影响,探讨了BD总皂苷减肥调脂作用的可能机制。方法：灌胃6周后,定位至大鼠左侧纹状体,于第3天进行大鼠纹状体微透析试验,将微透析液分别注入高效液相-电化学系统,测定各组纹状体透析液中单胺类神经递质五羟色胺（5-HT）、五羟基吲哚乙酸（5-HIAA）的含量。结果：与肥胖模型组相比,BD总皂苷高剂量组、低剂量组和阳性对照组纹状体透析液中5-HT、5-HIAA的含量升高,具有统计学意义（P〈0.05或P〈0.01）,说明BD总皂苷减肥作用可能与5-HT的含量升高有关。结论：BD总皂苷具有减肥和调节血脂作用,其作用可能与通过抑制5-HT、的再摄取而减少食物摄入有关。

Effects of Total Saponins From bd on Striatum Extracellular Levelsi of 5-HT and 5-HIAA in the Diet-induced Obese Rats

Objective：Aim To examine the effect of BD saponins on striatum extracellular levels of monoamine neurotransimtter in the Diet-Induced obese rats and its possible mechaninisms of the weight-Reducing and lipid regulating effect. Methods：Administering intragastrically to obese rats for six weeks,fixed the left striatum on rats.after 3d the striatal extracellular fluids were gained by brain microdialysis.Detected the levels of monoamine neurotransimtter of 5-HT、5-HIAA in each rat s. Results：Compared with the group of model,the levels of 5-HT、5-HIAA were increased in the striatum dialysate of BD the group of total saponins high dose、low-dose group and positive control,having statistical significance（P〈0.05 or P〈0.01）.Indicated the effect of weight-reducing of BD total saponins is possible relevant to the increasing of 5-HT. Conclusion：BD total saponins have the effect on weight-Reducing and lipid regulating,its possible mechaninisms could relate to restrain the reuptake of 5-HT 、5-HIAA so that it could decrease food intake.