早期动脉瘤模型大鼠形成过程中血管壁基质结构蛋白的表达

背景：基质蛋白是构成血管壁的必不可少的重要成分，为血管壁的完整性和血管壁细胞发挥生理功能提供了必要的框架，并参与对细胞和平滑肌的调控。 目的：构建早期动脉瘤模型大鼠评价基质结构蛋白在形成过程中的表达差异性。 方法：将28只健康雄性SD大鼠随机分为2组：对照组(n=8)和模型组(n=20)。模型组大鼠以结扎左侧颈总动脉和右侧肾肾动脉致高血压方法建立脑动脉瘤模型；对照组大鼠仅暴露左侧颈动脉分叉和双侧颈动脉。模型组大鼠分别于造模后15，30 d处死，取大鼠右侧大脑前动脉和嗅动脉的分叉处部分组织进行免疫组化染色，分析纤维连接蛋白、α-平滑肌肌动蛋白和Ⅲ型胶原蛋白的表达。 结果与结论：与对照组相比，造模后30 d时模型组大鼠右侧大脑前动脉和嗅动脉的分叉处部分中纤维连接蛋白表达水平差异无显著性意义(P > 0.05)，而Ⅲ型胶原和α-平滑肌肌动蛋白表达明显减少(P < 0.05)。提示大鼠早期脑动脉瘤形成过程中的结构蛋白表达存在差异性并发生动态变化，血管壁基质结构蛋白降解是动脉瘤形成主要机制之一。 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接：

Expression of matrix structural proteins in the vessel wall of rat models during the early aneurysm formation

BACKGROUND:Matrix protein is an essential component of the vascular wall, provides a necessary frame for the integrity of the vessel wall and physiological function of vascular wall cells, and regulates cells and smooth muscle. OBJECTIVE:To construct rat model of early aneurysm, and to evaluate differences in the expression of matrix structural proteins during cerebral aneurysm formation. METHODS:Twenty-eight healthy male Sprague-Dawley rats were randomized into control group (n=8) and model group (n=20). Aneurysm model was established by ligation of the left common carotid artery and right renal artery-induced hypertension in the model group. In the control group, only the left carotid artery bifurcation and bilateral carotid were exposed in rats. Rats in the model group were sacrificed at 15 and 30 days after model establishment. Right anterior cerebral artery in rats and olfactory artery bifurcation received immunohistochemical staining. The expressions of fibronectin, α-smooth muscle actin and collagen III were analyzed. RESULTS AND CONCLUSION:Compared with the control group, no significant difference in fibronectin expression was detected in right anterior cerebral artery and olfactory artery bifurcation in rats of the model group at 30 days after model establishment (P > 0.05). However, α-smooth muscle actin and collagen III expressions were significantly reduced (P < 0.05). These data confirmed that expression of structural proteins had differences and dynamic changes during early aneurysm formation in rats. Degradation of matrix structural protein in cerebral artery may be one of the key mechanism of aneurysm formation.