糖尿病牙周炎模型大鼠的牙槽骨丧失

背景：糖尿病牙周炎大鼠与单纯慢性牙周炎大鼠以及正常对照组大鼠在不同时间段内体质量、血糖、牙周组织的变化对牙槽骨丧失程度的影响不同。目的：建立糖尿病牙周炎模型大鼠和慢性牙周炎模型大鼠，观察糖尿病加重牙周炎大鼠槽骨吸收的破坏程度。方法：选用8周龄SPF级雄性SD大鼠52只，随机分为糖尿病牙周炎组、慢性牙周炎组以及正常对照组。糖尿病牙周炎组大鼠按采用注射链脲佐菌素+牙周结扎的方法建立糖尿病牙周炎模型大鼠。慢性牙周炎组大鼠采用牙周结扎并不断加压的方法建立慢性牙周炎大鼠模型；正常对照组大鼠正常喂养。各组大鼠分别于结扎后1，2，3，4周，观察各组大鼠牙周组织变化，取上颌骨牙槽骨标本，在体视显微镜下观察各组大鼠牙槽骨丧失值程度。结果与结论：钢丝结扎后1，2，3，4周大鼠牙槽骨丧失程度：糖尿病牙周炎组>慢性牙周炎组>正常对照组(P < 0.05)。结果证实，实验成功建立糖尿病牙周炎和慢性牙周炎动物模型，糖尿病可加重牙周炎牙周组织破坏，牙槽骨丧失增加。中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接：

Alveolar bone loss in rat models of diabetic periodontitis

BACKGROUND:Body weight, blood sugar, and the change of periodontal tissue in different time period of diabetic periodontitis rats, simple chronic periodontitis rats and normal rats have different influence on the degree of alveolar bone loss. OBJECTIVE:To establish rat models of diabetic periodontitis and chronic periodontitis, and to further clarify diabetes-increased degree of alveolar bone resorption in periodontitis rats METHODS:Fifty-two eight-week-old specific-pathogen-free male Sprague-Dawley rats were randomly divided into diabetic periodontitis group, chronic periodontitis group and normal control group. In the diabetic periodontitis group, rat models of diabetic periodontitis were established by injection of streptozotocin + periodontal ligation. In the chronic periodontitis group, rat models of chronic periodontitis were established by periodontal ligation and constant pressure. In the normal control group, rats were fed normally. At 1, 2, 3 and 4 weeks after ligation, changes in periodontium of rats of each group were observed. The maxilla alveolar bone specimens were taken. The degree of alveolar bone loss value in rats of each group was observed under a stereomicroscope. RESULTS AND CONCLUSION:At 1, 2, 3 and 4 weeks after ligation with steel wire, the degree of rat alveolar bone loss was as follows: diabetic periodontitis group > chronic periodontitis group > normal control group (P < 0.05). Results verified that animal models of diabetic periodontitis and chronic periodontitis were successfully established. Diabetes can increase the destruction to periodontal tissue with periodontitis, and increase alveolar bone loss.