不同程度脊髓背侧压迫损伤模型大鼠脊髓损伤致伤器的设计

背景：随着对脊髓损伤研究的不断深入，国内外学者不断设计出各种脊髓损伤模型，主要包括脊髓挫伤、重物坠击、脊髓压迫、化学灼伤、放射性损伤及激素横断半横断损伤等，然而各种制备方法都存在一定的局限。 目的：设计脊髓损伤致伤器并建立不同程度脊髓损伤动物模型。 方法：实验自行设计包括致伤部件及传动装置的脊髓损伤致伤器，利用不同致伤质量m1=10 g，m2=20 g，m3=30 g及时间T1=3 s，T2=5 s，造成不同质量×时间暨不同程度SD大鼠脊髓背侧压迫损伤，分为m1T1，m2T1，m3T1，m1T2，m2T2，m3T2共6组，并设假手术组进行对照。 结果与结论：造模后各实验组BBB评分均低于假手术组(P < 0.01)，m1T1组与其他各实验组相比差异有显著性意义(P < 0.01)，建模后8周m1T2组BBB评分高于与m2T2组及m3T2组(P < 0.05)。建模后8周各组体感诱发电位和运动诱发电位潜伏期明显长于假手术组(P < 0.01)。除了m1T1组与m1T2组外，其他各组间建模后体感诱发电位潜伏期均明显延长(P < 0.05)。所有组建模后运动诱发电位潜伏期均明显延长(P < 0.05)。结果证实，实验所设计的脊髓损伤致伤器可制备出不同程度的脊髓背侧压迫损伤动物模型。 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接：

Design of an injury device to establish spinal cord dorsal compression injury models in rats

BACKGROUND: With the development of spinal cord injury study, different methods of establishing spinal cord injury models have emerged, including spinal cord contusion, falling weight, spinal compression, chemical burn, radiation, hormone, spinal transection and hemi-section. However, lots of them are not perfect enough. OBJECTIVE: To design the injury device of spinal cord injury and establish different degrees of spinal cord injury models.  METHODS:To design the device of producing spinal cord injury and establish different degrees of spinal cord dorsal compression injury in Sprague-Dawley rats by various weights (m1=10 g, m2=20 g, m3=30 g) and time points (T1=3 s, T2=5 s). Rats were randomly divided as m1T1, m2T1, m3T1, m1T2, m2T2 and m3T2 groups. While sham group was also made. RESULTS AND CONCLUSION: Basso-Beattie-Bresnahan (BBB) score in injury groups decreased significantly after operation, when compared with the sham group (P < 0.01). The m1T1 group showed no significant difference in BBB score from other groups (P < 0.01). The BBB score of m1T2 group was significant higher than m2T2 group and m3T2 group at 8 weeks after operation (P < 0.05). The somatosensory evoked potential and motion evoked potential of injury groups were longer than sham group at 8 weeks after operation (P < 0.01). The motion evoked potential of each injury groups were significantly longer after operation (P < 0.05). The somatosensory evoked potential was significantly longer in injury groups, except m1T1 and m1T2 groups (P < 0.05). The self-designed device can be applied to establish different degrees of spinal cord injury models.