转染胶质细胞源性神经营养因子诱导神经干细胞分化

背景：神经干细胞为神经系统功能重建和神经再生提供了一条新的途径，解决其定向诱导分化问题仍是目前的研究热点。目的：探讨转染胶质细胞源性神经营养因子基因诱导大鼠胚胎神经干细胞向神经元和多巴胺能神经元分化的作用。方法：构建PcDNA3-GDNF-GFP表达质粒，用脂质体介导该质粒转染大鼠胚胎神经干细胞并进行诱导分化，荧光显微镜观察转染情况，免疫荧光染色检测β微管蛋白Ⅲ和酪氨酸羟化酶表达。结果与结论：胶质细胞源性神经营养因子基因转染后3 d，可观察到细胞呈绿色荧光细胞球状。诱导分化7 d，神经干细胞分化为神经元以及多巴胺神经元的比例明显增高。结果表明胶质细胞源性神经营养因子基因可以促进神经干细胞向神经元以及多巴胺能神经元分化。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Differentiation of neural stem cells induced by glial cell line-derived neurotrophic factor

BACKGROUND: The use of neural stem cells provides a new approach for nervous system functional reconstruction and nerve regeneration. How to solve the induced differentiation of neural stem cells is still a research hotspot. OBJECTIVE: To investigate the differentiation of rat neural stem cells into neurons and dopaminergic neurons under induction of glial cell-line derived neurotrophic factor gene.METHODS: PcDNA3-GDNF-GFP plasmids were constructed and transferred via lipidosome into rat embryonic neural stem cells. Differentiation of neural stem cells after transfection was identified under a fluorescence microscope. Immunofluorescent staining was used to detect expression of β-tubulin III and tyrosine hydroxylase. RESULTS AND CONCLUSION: At 3 days after transfection, green fluorescence was observed in the transfected cells that were globular. At 7 days after transfection, the proportion of neural stem cells differentiating into neurons and dopaminergic neurons were significantly increased. These results indicate that the glial cell-line derived neurotrophic factor gene can induce the differentiation of neural stem cells into neurons and dopaminergic neurons.