雌激素受体基因多态性与老年妇女的骨密度

背景：雌激素受体α基因多态性与骨质疏松的发生相关有一定的关系，但是对于危险基因型的研究还存在商榷余地。目的：分析雌激素受体基因多态性与老年妇女骨密度的相关性。方法：选择检查的老年健康妇女120例，提取全血基因组DNA，选择限制性内切酶PvuⅡ和XbaⅠ进行酶切，分析基因型的分布与频率。同时选择双能X射线骨密度仪测股骨颈、大转子及Ward三角处的骨密度值。结果与结论：XbaⅠ酶切基因型XX 6例，Xx 78例，xx 36例；PvuⅡ酶切PP 32例，Pp 50例，pp 38例。不同基因型老年妇女的年龄、绝经年龄与体质量指数值对比差异无显著性意义(P > 0.05)。PvuⅡ酶切PP基因型妇女的大转子与ward三角处的骨密度值明显大于Pp及pp妇女(P < 0.05)；而XbaⅠ酶切基因多态性在老年妇女中股骨颈、大转子与Ward三角的骨密度均无显著差异(P > 0.05)。说明雌激素受体基因多态性与老年妇女骨密度有一定的相关性，P等位基因对老年妇女大转子与Ward三角处的骨密度的维持有一定作用。中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接：

Correlation between estrogen receptor gene polymorphism and bone mineral density in elderly women

BACKGROUND: Estrogen receptor α gene polymorphisms is certainly associated with osteoporosis, but there is still questionable for the study of risk genotypes.OBJECTIVE: To analyze the correlation between estrogen receptor gene polymorphism and bone mineral density in elderly women. METHODS:Totally 120 healthy elderly women were enrolled to extract the whole blood genomic DNA that was digested using Pvu II and Xba I, the distribution and frequency of genotypes were analyzed. Meanwhile, dual-energy X-ray absorptiometry measurements of bone mineral density with the femoral neck, greater trochanter and Ward’s triangle and were done.RESULTS AND CONCLUSION: Xba I digested genotypes included the XX in 6 cases, Xx in 78 cases, xx in 36 cases; Pvu II digested genotypes included the PP in 32 cases, Pp in 50 cases, pp in 38 cases. The age, menopause age and body mass index showed no differences among the different genotypes of elderly women  (P > 0.05). The bone mineral density values in the trochanter and Ward’s triangle at Pvu II digested PP genotype were significantly greater than those at Pp and pp genotypes (P < 0.05); while no significant differences were found in the bone mineral density values at different Xba I digested genotypes in elderly women (P > 0.05). These findings suggest that estrogen receptor gene polymorphism has certain correlation with the bone mineral density in elderly women, P alleles has certain effect to maintain the bone mineral density of the trochanter and Ward’s triangle in elderly women.