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痰中结核杆菌的不同检测方法比较及临床应用
引用本文:孙庆,刘成永,钱秀梅,侯远沛,王双. 痰中结核杆菌的不同检测方法比较及临床应用[J]. 基层医学论坛, 2011, 15(10): 291-292
作者姓名:孙庆  刘成永  钱秀梅  侯远沛  王双
作者单位:徐州市传染病医院,江苏,徐州,221004
摘    要:
目的 探讨不同实验方法检测结核杆菌的阳性率以及在临床上的应用价值.方法 对380份痰标本分别采用直接涂片、夹层杯离心涂片集菌法、罗氏培养法以及PCR方法检测,并对阳性检出率进行比较.结果 PCR方法阳性率最高,但存在假阳性和假阴性;罗氏培养法为金标准,但周期较长;夹层杯离心涂片集菌法痰涂片阳性率为49.2%,直接涂片法...

关 键 词:  结核杆菌  夹层杯离心涂片集菌法  直接涂片

Comparison of different detection methods to mycobacterium tuberculosis in sputum and its clinical application
Sun Qing,Liu Chengyong,Qian Xiumei,et al.. Comparison of different detection methods to mycobacterium tuberculosis in sputum and its clinical application[J]. Public Medical Forum Magazine, 2011, 15(10): 291-292
Authors:Sun Qing  Liu Chengyong  Qian Xiumei  et al.
Affiliation:Sun Qing,Liu Chengyong,Qian Xiumei,et al.The Infectious Diseases Hospital of Xuzhou City,Xuzhou,Jiangsu 221004
Abstract:
Objective To study the positive rate of mycobacterium tuberculosis by different experimental methods and its clinical value.Methods Compared the detected positive rate of 380 sputum specimens by direct smear method,centrifuging the liquored sputa and collecting bacteria method,L-J medium method,and TB-PCR method.Results The detected positive rate of TB-PCR method was the highest but existed false positive and false negative.L-J medium method in detection was the diagnostical golden standard,but the cycle of...
Keywords:Sputum Bacillus tuberculosis Centrifuging the liquored sputa and collecting bacteria Direct smear  
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