血管内皮生长因子（VEGF）及其可溶性受体在早期复发性流产患者中的表达及临床意义

目的：探讨血管内皮生长因子（VEGF）及其可溶性受体（silt．1）与复发性流产（RSA）的相关性。方法：应用酶联免疫吸附试验（ELIsAl技术和免疫组织化学方法分别检测30例RSA患者血清和绒毛中VEGF及silt-1的表达情况，并以同期因非意愿妊娠要求行人工流产术的正常早孕妇-k-（n=30）为对照，比较VEGF及silt-1表达的临床意义。结果：RSA组血清VEGF（2637．50±2145．09ng／L）、silt—l蛋白（13796．67±ll917．08ng／L）表达水平均高于对照组（分别为982．67±675．47ng／L和3540．67±2989．80ng／L）（P〈O．051：RSA组绒毛组织vEGF和silt-1的表达阳性率（分别为76．7％和96．7％）均高于对照组（分别为50．0％和66．7％）（P〈O．05）。结论：RSA患者血清和绒毛中VEGF、silt-1表达程度升高，可能是导致孕早期胚胎停育的原因之一。

Expression and Clinical Significance of VEGF and VEGF Soluble Receptor-1 in Patients of Early Recurrent Abortion

Objective： To explore the relationship between early recurrent spontaneous abortion （RSA） and vascular endothelial growth factor （VEGF）, VEGF soluble receptor （silt-1）. Methods： Serum levels of VEGF and silt-1 in 30 healthy early pregnancy controls of voluntary induced abortion and 30 patients of RSA were detected by ELISA. Histological localization and quantitative analysis of VEGF and silt-1 in their chorionic villi were detected by immunohistochemistry after induced abortion. Results： The expression levels of VEGF and silt-1 in both serum and ehorionic villus tissue in RSA patients were significantly higher than those of the healthy controls （P〈0.05）. Serum levels of BEGF （2 637.50±2 145.09 ng/L） and silt-1 （13 796.67 ± 11 917.08 ng/L） in RSA group were significantly higher than those in the control （982.67 ± 675.47 ng/L, 3 540.67 ± 2 989.80 ng/L）（P〈0.05）. The positive rates of VEGF and sft-1 in chorionic villi tissues of RSA patients （76.7%, 96.7%） were also significantly higher than those of the control （50.0%, 66.7%）（P〈0.05）. Conclusion： The high expression level of VEGF and silt-1 protein in both serum and chorionic villus tissue in RSA patients may be involved in the pathogenesis of embryo growth arrest.