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| 黄芪注射液对体外培养视网膜神经节细胞的保护作用 | |
| 引用本文： | 廖良,孔莹莹,韦企平,周剑,徐铭谦,孙艳红. 黄芪注射液对体外培养视网膜神经节细胞的保护作用[J]. 眼科新进展, 2010, 30(12) |
| 作者姓名： | 廖良 孔莹莹 韦企平 周剑 徐铭谦 孙艳红 |
| 作者单位： | 北京中医药大学东方医院眼科; |
| 摘 要： | 目的 视神经由视网膜神经节细胞(retinal ganglion cells,RGCs)轴突构成,一旦损伤难以再生,视神经保护是治疗视神经疾病的关键,本研究拟评价黄芪注射液的视神经保护作用.方法 体外培养RGCs,采用谷氨酸作为细胞凋亡诱导剂建立RGCs凋亡模型,并用不同浓度黄芪注射液加入细胞培养基进行干预,分别用MTT和流式细胞检测的方法进行细胞存活率及细胞凋亡相关检测.结果 黄芪注射液加入DMEM-F12培养基共培养RGCs 24 h后,MTT检测结果示终浓度为(0.10～0.40)×103g·L-1时黄芪注射液均可促进RGCs存活,其中0.20×103g·L-1、0.30×103g·L-1浓度促生长作用最好(P=0.040、0.005,n=10),谷氨酸浓度为3.2 mmol·L-1时可造成理想的RGCs凋亡模型,0.20×103g·L-1黄芪注射液可促进谷氨酸损伤的RGCs存活(P=0.08,n=10)并减少其细胞凋亡率(P=0.36,n=4).结论 黄芪注射液作为视神经保护中药制剂对RGCs具有一定保护作用,黄芪对RGCs的保护作用很可能跟抑制谷氨酸兴奋性毒性作用有关. |
| 关 键 词： | 黄芪注射液 视网膜神经节细胞 细胞凋亡 |
Protective effect of Huangqi injection on retinal ganglion cell cultured in vitro |
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| LIAO Liang,KONG Ying-Ying,WEI Qi-Ping,ZHOU Jian,XU Ming-Qian,SUN Yan-Hong. Protective effect of Huangqi injection on retinal ganglion cell cultured in vitro[J]. Recent Advances in Ophthalmology, 2010, 30(12) | |
| Authors: | LIAO Liang KONG Ying-Ying WEI Qi-Ping ZHOU Jian XU Ming-Qian SUN Yan-Hong |
| Affiliation: | LIAO Liang,KONG Ying-Ying,WEI Qi-Ping,ZHOU Jian,XU Ming-Qian,SUN Yan-Hong From the Eye Department Dongfang Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100078,China |
| Abstract: | Objective Optic nerve was constructed with axons of retinal ganglion cells(RGCs),and it was hard to regenerate after injury.Protection of optic nerve was the key to treat diseases of optic nerve.This study was to evaluate the neuroprotective effect of Huangqi injection.Methods RGCs was cultured in vitro.Glutamic acid was treated as cell apoptotic inductor to build RGCs apoptotic models.Different concentrations of Huangqi injection were added to cell culture medium to interfere.MTT and flow cytometry were us... |
| Keywords: | Huangqi injection retinal ganglion cells cell apoptosis |
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