制作缺血性中风气虚血瘀证大鼠模型的实验研究

目的 探索建立缺血性中风气虚血瘀证大鼠模型的制作方法和评价体系。

Establishment of Rat Model of Ischemic Stroke with Qi Deficiency and Blood Stasis Syndrome and Its Evaluation System

Objective To probe into the establishing method and the evaluating system for rat model of ischemic stroke with qi deficiency and blood stasis syndrome （QDBS）. Methods A rat model of ischemic stroke of QDBS was established by continual exhaustive swimming followed with middle cerebral artery occlusion （MCAO）, and evaluated by analyzing the changes of exterior signs, tongue figure, hemorrheologic characters and brain histomorphology in the model rats. Results The model rats showed a state of QDBS in the course of continual exhaustive swimming, such as slower weight gain, postponed food intake, darker tongue and longer sublingual veins; and presented the characteristics of cerebral ischemia with QDBS syndrome after MCAO, they were inactive, weak, and hemiplegic, with dark purple tongue and longer blue sublingual veins. Moreover, hem- orrheologic examinations showed blood hyperviscosity and high platelet aggregation rate, and histomorphologic examinations showed a special figure of ischemic changes. Conclusion Continual exhausting swimming followed by MCAO is a method for establishing a rat model of ischemic stroke with QDBS syndrome, and its evaluating system could be constituted by multiple criteria, including exterior signs, tongue figure, hemorrheologic and histomorphologic indexes, etc.