The Papain Inhibitor (SPI) of Streptomyces mobaraensis Inhibits Bacterial Cysteine Proteases and Is an Antagonist of Bacterial Growth |
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| Authors: | Stephan Zindel Wendy E. Kaman Sabrina Fr?ls Felicitas Pfeifer Anna Peters John P. Hays Hans-Lothar Fuchsbauer |
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| Affiliation: | Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Darmstadt, Germanya;Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlandsb;Department of Biology, Technische Universität Darmstadt, Darmstadt, Germanyc |
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| Abstract: | A novel papain inhibitory protein (SPI) from Streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (Staphylococcus aureus SspB) and culture supernatants (Porphyromonas gingivalis, Bacillus anthracis). Further, growth of Bacillus anthracis, Staphylococcus aureus, Pseudomonas aeruginosa, and Vibrio cholerae was completely inhibited by 10 μM SPI. At this concentration of SPI, no cytotoxicity was observed. We conclude that SPI inhibits bacterial virulence factors and has the potential to become a novel therapeutic treatment against a range of unrelated pathogenic bacteria. |
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