尿毒清胶囊对梗阻性肾病大鼠肾间质纤维化的影响

目的：观察尿毒清胶囊对梗阻性肾病大鼠肾间质纤维化的影响，探讨其对大鼠肾间质纤维化的改善作用及其机制。方法：采用单侧输尿管结扎（UUO）大鼠模型，中药组以尿毒清灌胃，依那普利组则以依那普利灌胃，空白组以蒸馏水灌胃，另设假手术组作正常对照。应用免疫组化法检测肾组织细胞因子转化生长因子-β（TGF-β）、磷酸化的Smads信号蛋白2／3（P-Smad2／3）和α-平滑肌肌动蛋白（α—SMA）在梗阻性肾病肾组织中的表达。结果：尿毒清胶囊、依那普利治疗可以明显抑制肾组织TGF-β1、P—Smad2／3的表达（P〈0．05），显著下调肾组织α-SMA的表达（P〈0．05）。结论：尿毒清胶囊与依那普利均可显著抑制大鼠梗阻性肾病α-SMA的表达，从而减轻肾间质纤维化，这一作用可能与其抑制肾组织TGF-β1、P-Smad2／3的表达有关。

Effect of Niaoduqing Capsule on Renal Tubulointerstitial Fibrosis in Rats with Obstructive Nephropathy

Objective： To investigate the effect of Niaoduqing Capsule on renal tubulointerstitial fibrosis in rats with obstructive nephropathy, and to explore its possible mechanism. Methods： Rat models were established by unilateral ureteral obstruction（UUO）. The rats were randomly divided into 4 groups., sham-operation group, model group, Niaoduqing Capsule group, enalapril group. Immunohistochemical staining was performed to investigate the expression of transforming growth factor- β1 （TGF-β1）, phosphorylated Smad2/3 and interstitial α -smooth muscle actin （α -SMA） in the renal tissue of the obstructed kidney. Results： Both Niaoduqing Capsule and enalapril significantly suppressed the activity of phosphrylated Smad2/3 and the expression of α -SMA in the obstructed kidney （P 〈 0.05）. Conclusion： Both Niaoduqing Capsule and Enalapril can significantly alleviate renal tubulointerstitial fibrosis by suppressing the expression of α-SMA in the obstructed kidney of rats, which may be associated with the inhibition of Smad2/3 activity.