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| Notch1突变对HSC体外分化为T/B淋巴细胞的影响 | |
| 引用本文： | 王磊,洪剑,王玉华,苗丽丽,许望翔,杨晓明,葛常辉. Notch1突变对HSC体外分化为T/B淋巴细胞的影响[J]. 中国免疫学杂志, 2011, 27(9): 804-808. DOI: 10.3969/j.issn.1000-484X.2011.09.010 |
| 作者姓名： | 王磊 洪剑 王玉华 苗丽丽 许望翔 杨晓明 葛常辉 |
| 作者单位： | 1. 解放军军事医学科学院放射与辐射医学研究所,北京,100850 2. 解放军第309医院放射科,北京,100090 3. 爱因斯坦医学院内分泌系,纽约,NY10461 |
| 基金项目： | 国家自然科学基金，北京市自然科学基金，教育部留学回国人员科研启动基金资助 |
| 摘 要： | 目的:探讨Notch1信号传递水平对小鼠造血干细胞(HSC)体外分化为T/B淋巴细胞的影响。方法:应用表达Notch1配体Delta1的基质细胞OP9-DL与来源于小鼠胎肝和骨髓的Lin-HSC在体外共培养,检测Notch1基因突变对HSC在体外分化后B淋巴细胞或者CD4+CD8+细胞的比例。结果:Notch1基因点突变后Notch1受体结构发生改变,和特异性抗体以及配体的结合能力降低,导致信号传递能力下降。Notch1突变后,胎肝和骨髓HSC向B淋巴细胞分化过程未受影响,但是向T淋巴细胞的分化过程受到明显抑制,其中Notch1突变对胎肝HSC分化能力的影响高于骨髓HSC,分别被抑制44%和34%。结论:Notch1突变导致Notch1信号水平降低,对HSC向B淋巴细胞分化没有影响,但是能抑制向T淋巴细胞的分化。 |
| 关 键 词： | Notch1 基因突变 OP9细胞 体外分化 |
Effects of Notch1 mutation on in vitro differentiation of hematopoietic stem cells into T/B lymphocytes |
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| WANG Lei,HONG Jian,WANG Yu-Hua,MIAO Li-Li,XU Wang-Xiang,YANG Xiao-Ming,GE Chang-Hui. Effects of Notch1 mutation on in vitro differentiation of hematopoietic stem cells into T/B lymphocytes[J]. Chinese Journal of Immunology, 2011, 27(9): 804-808. DOI: 10.3969/j.issn.1000-484X.2011.09.010 | |
| Authors: | WANG Lei HONG Jian WANG Yu-Hua MIAO Li-Li XU Wang-Xiang YANG Xiao-Ming GE Chang-Hui |
| Affiliation: | WANG Lei,HONG Jian,WANG Yu-Hua,MIAO Li-Li,XU Wang-Xiang,YANG Xiao-Ming,GE Chang-Hui.Beijing Institute of Radiation Medicine,Beijing 100850,China |
| Abstract: | Objective:To investigate the effects of Notch1 signal transduction level on hempatopoietic stem cells(HSCs) in vitro differentiation into T/B lymphocytes.Methods:The HSCs from fetal liver cells or bone marrow from mice that carrying a Notch1 point mutation within its ligand binding domain were co-cultured with stromal OP9 or OP9-DL1 that can express Delta1.Proportions of B lymphocytes or CD4+CD8+ cells were analyzed by FACS.Results:Notch1 mutation may change conformation of ligand binding domain of Notch1 r... |
| Keywords: | Notch1 |
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