乌骨藤提取物对人结肠腺癌细胞增殖的实验研究

目的:研究乌骨藤提取物对体外培养的人肠癌细胞(Lovo)增殖的影响。方法:用两种方法(A、B)制备乌骨藤提取物,分别采用不同浓度作用Lovo细胞,通过细胞形态学和MTT法检测乌骨藤提取物对Lovo细胞增殖的影响。结果:乌骨藤提取物A对细胞的最大无毒浓度约为0.648 mg/mL;药物浓度在0.389、0.648、1.08、1.8、3 mg/mL时的抑制率分别为6.58%、8.24%、13.02%、59.69%、66.23%,用回归法求出乌骨藤提取物A对Lovo细胞增殖的半数抑制浓度(IC_(50))为1.625 mg/mL。乌骨藤提取物B对细胞的最大无毒浓度约为0.648 mg/mL,药物浓度在0.389、0.648、1.08、1.8、3 mg/mL时的抑制率分别为14.49%、15.48%、29.69%、63.26%、64.05%,用回归法求出乌骨藤提取物B对Lovo细胞增殖的半数,IC_(50)为1.84 mg/mL。结论:乌骨藤提取物对体外培养的Lovo细胞的增殖有明显抑制作用。

Study of glaucescent fissistigma root extractive on multiplication of Lovo cells (human colon adenocarcinoma)

AIM:To investigate the effects of the ex- tracts from Wu Guteng on the multiplication of Lovo cells (human colon cancer).METHODS:Two methods(A, B)are used to prepare the medicine solution from the liq- uids withdrawn of Wu Guteng,which is diluted to differ- ent densities to incubate with Lovo cells for 24 h and 48 h.Cytomorphology and the MTF method are used to test the effects of the liquids withdrawn from Wu Guteng on the multiplication of the Lovo cells.RESULTS:The maximal nontoxic density of the extractive from Wu Guteng by method A to Lovo cells was about 0.648 mg/mL.When the medicine density were 0.389,0.648, 1.08,and 1.8 and 3 mg/mL,the inhibiting rates were 6.58%,8.24%,13.02%,59.69% arid 66.23% re- spectively.The IC_(50)of extractive from Wu Guteng by method A to Lovo cells was 1.625 mg/mL.The maximal nontoxic density of extractive from Wu Guteng by method A ws about 0.648 mg/mL When the density of the extrac- tive from Wu Guteng by method B to Lovo cells were 0.389,0.648,1.08,and 1.8 3 mg/mL,the inhibiting rates were 14.49%,15.48%,29.69%,63.26% and 64.05% respectively.The IC_(50)of extractive from Wu Guteng by method B was 1.84 mg/mL.CONCLUSION: The extracts from Wu Guteng inhibite the muhiplication of the cancer cells of colon glands.