| 多发性硬化患者造血干/祖细胞及其亚群富集纯化的研究 |
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| 引用本文： | 秦超,莫雪安,陆锐,凌莉,莫武宁. 多发性硬化患者造血干/祖细胞及其亚群富集纯化的研究[J]. 医学争鸣, 2004, 25(3): 233-235 |
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| 作者姓名： | 秦超 莫雪安 陆锐 凌莉 莫武宁 |
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| 作者单位： | 广西医科大学第一附属医院,神经内科,广西,南宁,530021;广西医科大学第一附属医院,检验科,广西,南宁,530021 |
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| 基金项目： | 广西自然科学基金 (桂科基 0 342 0 0 8 1 ) |
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| 摘 要： | 目的：获得高纯度造血干／祖细胞(HSC／HPC)及其亚群，用于多发性硬化(MS)造血干细胞移植治疗和生物学特性的研究．方法：用免疫磁珠法(MiniMACS)从MS患者骨髓中富集CD34^ 细胞后再用荧光激活细胞分选(fluorescent acti—vated cell sorter，FACS)纯化CD34^ ／CD38^ 和CD34^ ／CD38^-细胞亚群．结果：MiniMACS分选的CD34^ 细胞群纯度达91％，FACS分选的CD34^ ／CD38^-和CD34^ ／CD38^ 两细胞亚群纯度可达98％以上．结论：MiniMACS和FACS两者结合可迅速大量纯化HSC／HPC及其重要细胞亚群，可用于临床MS造血干细胞移植、基因治疗和研究HSC／HPC及其亚群生物学特征．
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| 关 键 词： | 造血干细胞 免疫磁珠法 流式细胞计量术 多发性硬化 |
| 文章编号： | 1000-2790(2004)03-0233-03 |
| 修稿时间： | 2003-10-08 |
Collection and purification of CD34+ hematopoietic stem (progenitor) cells and its sub-groups from patients with multiple sclerosis |
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| QIN Chao ,MO Xue An ,LU Rui ,LING Li ,MO Wu Ning. Collection and purification of CD34+ hematopoietic stem (progenitor) cells and its sub-groups from patients with multiple sclerosis[J]. Negative, 2004, 25(3): 233-235 |
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| Authors: | QIN Chao MO Xue An LU Rui LING Li MO Wu Ning |
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| Affiliation: | QIN Chao 1,MO Xue An 1,LU Rui 1,LING Li 1,MO Wu Ning 2 1Department of Neurology,2Department of Clinical Laboratories,First Affiliated Hospital,Guangxi Medical University,Nangning 530021,China |
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| Abstract: | AIM: To collect and purify the bone marrow CD34 + hematopoietic stem cells and its sub groups from the patients with multiple sclerosis. METHODS: CD34 + cells were collected and purified by means of imunomagnetic beads on MiniMACS device. Flow cytometry was emplyed to determine percentage of CD34 + cells, CD34 +/CD38 - cells and CD34 +/CD38 + cells before and after miniMACS. RESULTS: The purity of CD34 + cells amounted to more than 91% after the purification. The purity of CD34 +/CD38 - cells and CD34 +/CD38 + cells amounted to more than 98% after fluorescent activated cell sorter (FACS). CONCLUSION: The combination of MiniMACS and FACS can sort effectively the bone marrow CD34 + cells and its sub groups so that they may be used for hematopoietic stem cell transplantation, gene therapy and biological study of hematopoietic stem cells/hematopoietic progenitor cells (HSC/HPC) and its sub groups. |
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| Keywords: | hematopoietic stem cells MiniMACS flow ytometry multiple sclerosis |
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