基于体内外相结合的中药大品种二次开发物质基础研究思路

中药大品种二次开发是中药现代化、科学化、标准化进程的重要环节之一。如何准确有效地识别原方有效物质基础是二次开发中最首要的难题，同时也是中药现代化发展的瓶颈。该文在目前已有的试验方法的基础上，根据中药复方多成分、复杂作用需要综合多学科的方法与技术协同研究的思路，提出基于体内外相结合的中药大品种二次开发物质基础研究思路，认为物质基础研究需要分为识别、筛选、验证3个环节，每个环节设计相应的体内、外的研究方法相互补充、相互验证，最终筛选正确可靠的复方物质基础。该思路为中药大品种二次开发以及复方物质基础研究提供参考。

Study thought of material basis of secondary development of major traditional Chinese medicine varieties on basis of combination of in vivo and in vitro experiments

The secondary development of major traditional Chinese medicine varieties is one of important links during the modernization, scientification and standardization of traditional Chinese medicines. How to accurately and effectively identify the pharmacodynamic material basis of original formulae becomes the primary problem in the secondary development, as well as the bottleneck in the modernization development of traditional Chinese medicines. On the basis of the existing experimental methods, and according to the study thought that the multi-component and complex effects of traditional Chinese medicine components need to combine multi-disciplinary methods and technologies, we propose the study thought of the material basis of secondary development of major traditional Chinese medicine varieties based on the combination of in vivo and in vitro experiments. It is believed that studies on material basis needs three links, namely identification, screening and verification, and in vivo and in vitro study method corresponding to each link is mutually complemented and verified. Finally, the accurate and reliable material basis is selected. This thought provides reference for the secondary development of major traditional Chinese medicine varieties and studies on compound material basis.