树鼩粪便细菌分离培养与鉴定

目的 了解人工饲养树鼩粪便菌群多样性,为树鼩的正常饲养繁殖和微生物质量控制标准化提供依据。方法 随机采集10份树鼩粪便样品,利用有氧及厌氧培养基进行细菌分离培养,提取细菌基因组DNA后PCR扩增16SrRNA基因并测序鉴定。结果 本实验从树鼩粪便样品中,经有氧培养分离鉴定出25株、12种细菌,经厌氧培养分离鉴定出25株、10种细菌,包括变形杆菌属、肠球菌属、埃希菌属、志贺菌属、葡萄球菌属、气单胞菌属、拉恩氏菌属、拉乌尔菌属、微小杆菌属、链球菌属、明串珠菌属。结论 树鼩肠道好氧菌及厌氧菌具有丰富的种属多样性,普通变形杆菌群、费格森埃希菌群和屎肠球菌群可能是树鼩肠道的主要寄生菌群。

Isolation, culture and identification of bacterial strains from tree shrews feces

Objective Study the fecal flora diversity of the tree shrew, to provide a basis data of fecal bacteria of feeding the tree shrew. Methods Ten tree shrews were used in this study. The Stools of the animals were respectively cultured with oxygen and without oxygen to isolate the bacterial. Then the PCR-amplified 16S rRNA of the bacterial was sequenced and analyzed. Results 25 bacterial strains belonging to ten bacterial species were isolated by anaerobic incubation, and 25 bacterial strains belonging to twelve bacterial species were isolated by aerobic incubation. Proteus vulgaris, Enterococcus faecalis, Escherichia fergusonii, Enterococcus faecium, Shigella flexneri, Shigella sonnei, Staphylococcus aureus, Aeromonas salmonicida subsp.masoucida, Rahnella aquatilis, Exiguobacterium aquaticum, Raoultella terrigena, and Escherichia coli were identified in this study. Conclusions There is a fecal flora diversity of the tree shrew, and the Proteus vulgaris, Escherichia fergusonii and Enterococcus faecium may be the major parasitic flora.