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| 肉制品中诱惑红的高效液相色谱测定法 | |
| 引用本文： | 张向红. 肉制品中诱惑红的高效液相色谱测定法[J]. 职业与健康, 2011, 27(12): 1363-1365 |
| 作者姓名： | 张向红 |
| 作者单位： | 河南省偃师市疾病预防控制中心检验科,471900 |
| 摘 要： | 目的建立肉制品中诱惑红的高效液相色谱测定方法。方法样品中的诱惑红色素经提取后在酸性条件下被聚酰胺粉吸附,在碱性条件下解吸附,以甲醇—0.02 mol/L乙酸铵溶液为流动相,梯度洗脱,C18柱分离,紫外检测器检测,波长254 nm,以相对保留时间定性,色谱峰面积定量。结果浓度与峰面积线性关系良好r=0.999 5,回收率90.0%～93.7%,相对标准偏差在1.25%～1.82%之间,最低检出浓度为0.001 g/kg。结论该方法操作简便、灵敏、准确,适用于肉制品中诱惑红、柠檬黄、胭脂红、日落黄等色素的同时检测。 |
| 关 键 词： | 高效液相色谱法 肉制品 诱惑红 |
Determination of Allura Red in Meat Products by High Performance Liquid Chromatography |
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| ZHANG Xiang-hong. Determination of Allura Red in Meat Products by High Performance Liquid Chromatography[J]. Occupation and Health, 2011, 27(12): 1363-1365 | |
| Authors: | ZHANG Xiang-hong |
| Affiliation: | ZHANG Xiang-hong(Yanshi Center for Disease Control and Prevention,Henan,471900,China) |
| Abstract: | [Objective]To establish the method for dectecting Allura Red in meat products by High Performance Liquid Chromatography(HPLC).[Methods]Allura Red was adsorbed by polyamide powder after extraction under acidic conditions,desorption in alkaline conditions,methanol-0.02 mol/L ammonium acetate as the mobile phase gradient elution for gradient elution,C18 column for separation and UV detector for detection,with wavelength 254 nm,the relative retention time was qualitative,chromatographic peak area quantification... |
| Keywords: | High Performance Liquid Chromatography(HPLC) Meat product Allura Red |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |