喉癌组织中IGFBP-rP1基因的甲基化及表达

目的：探讨喉癌中胰岛素样生长因子结合蛋白一相关蛋白1（IGFBP—rP1）基因启动子区甲基化与蛋白表达之间的关系。方法：采用甲基化特异性PCR方法和免疫组织化学的方法分别检测45例喉癌组织及18例癌旁组织中IGFBP-rP1基因的甲基化状态和蛋白表达情况。结果：喉癌组织IGFBP—rP1启动子区甲基化率为33．3％（15／45），相应癌旁组织甲基化率为5．6％（1／18），喉癌组织中IGFBP—rP1基因甲基化率明显高于癌旁组织（P〈0．05）。喉癌组织中IGFBP—rP1蛋白表达显著低于癌旁正常组织（P％0．05），且与其启动子区甲基化状态呈负相关。结论：IGFBP—rP1基因启动子区甲基化导致基因沉默可能是喉癌发生的机制之一。

Expression and methylation status of IGFBP-rP1 gene in laryngocarcinoma

To investigate the relationship between the promoter methylation and protein expression of insulin-like growth factor binding protein-related protein 1 （IGFBP-rP1） in laryngeal squamous cell carcinoma （LSCC）. Method： Methylation specific PCR （MSP） approach and immunohistochchemistry methods were used to examine the methylation status and protein expression of IGFBP-rP1 in 45 samples of laryngeal carcinoma and 18 samples of tissues beside cancer . Result： For the promoter site, methylation frequency of IGFBP-rP1 in tumor specimens（33.3$, 15/45） was significantly higher than that in corresponding nomal tissues（5.6%, 1/18）（P〈0.05）. The protein expression of IGFBP-rP1 in tumor tissues was significantly higher than that in corresponding normal tissues（P〈0.05） and was inversely correlated with its methylation status of promoter. Conclusion： Epige- netic silencing of IGFBP-rP1 gene expression by promoter hypermethylation may play an important role in LSCC.