Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus |
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| Authors: | Andreas?Lucht mailto:andreaslu@gmx.de" title=" andreaslu@gmx.de" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Roland?Grunow,Christian?Otterbein,Peggy?M?ller,Heinz?Feldmann,Stephan?Becker |
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| Affiliation: | (1) Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany;(2) Institute of Virology, Philipps-University Marburg, Robert-Koch-Strasse 17, 35037 Marburg, Germany;(3) Present address: Authority of the district of Rosenheim, Public Veterinary Office, Am Klafferer 3, 83043 Bad Aibling, Germany;(4) Present address: Special Pathogens Program, National Microbiology Laboratory, Health Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Canada |
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| Abstract: | ![]()
Ebola virus (EBOV) causes severe outbreaks of Ebola hemorrhagic fever in endemic regions of Africa and is considered to be of impact for other parts of the world as an imported viral disease. To develop a new diagnostic test, monoclonal antibodies to EBOV were produced from mice immunized with inactivated EBOV species Zaire. Antibodies directed against the viral glycoprotein GP were characterized by ELISA, Western blot and immunofluorescence analyses. An antigen capture ELISA was established, which is specific for EBOV-Zaire and shows a sensitivity of approximately 103 plaque-forming units/ml. Since the ELISA is able to detect even SDS-inactivated EBOV in spiked human sera, it could complement the existing diagnostic tools in the field and in routine laboratories where high containment facilities are not available. |
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| Keywords: | Ebola virus Ebola hemorrhagic fever Monoclonal antibodies Antigen capture ELISA |
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