A Simple Method for Isolation of Cardiomyocytes from Adult Rat Heart |
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| Authors: | M. V. Egorova S. A. Afanas'ev S. V. Popov |
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| Affiliation: | (1) Institute of Cardiology, Tomsk Research Center, Siberian Division, Russian Academy of Medical Sciences, Russia |
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| Abstract: | A simple, economic, and sparing method for isolation of cardiomyocytes from adult rat heart is proposed. Ultrastructure of cardiomyocytes from suspension of freshly isolated cells was studied by light and transmission electron microscopy. The isolated cardiomyocytes were viable, had characteristic shape and size, and retained their normal structure. __________ Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 140, No. 9, pp. 357–360, September, 2005 |
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| Keywords: | cardiomyocytes collagenase pronase heart perfusion enzymatic treatment of the heart |
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