免疫抑制小鼠系统性白色念珠菌感染模型建立的研究

目的:建立免疫抑制小鼠系统性白色念珠菌感染模型,为感染性疾病的研究和治疗提供科学依据。方法:给予昆明种正常小鼠腹腔内一次性注射环磷酰胺(CY)200 mg/kg,观察其对小鼠一般状况和白细胞数的影响。继之,分别给予前述小鼠尾静脉接种1.5×105/ml(低浓度组)、1.5×106/ml(中浓度组)、1.5×107/ml(高浓度组)的白色念珠菌菌液,建立感染模型。结果:正常小鼠腹腔注射CY后,各组均出现喜静、少动、消瘦、饮食明显减少、多尿、皮毛欠光滑等现象以及白细胞数的降低。小鼠尾静脉分别接种1.5×105/ml(低浓度组)、1.5×106/ml(中浓度组)、1.5×107/ml(高浓度组)浓度的白色念珠菌菌液后,连续观察3周。低浓度组小鼠死亡率为20%,中浓度组死亡率为50%,高浓度组则全部死亡。组织真菌培养证实白色念珠菌已经侵染小鼠内脏,证明感染模型建立成功。结论:CY能够有效降低小鼠白细胞数,导致免疫力低下。在此基础上经尾静脉接种合适浓度的白色念珠菌可建立小鼠系统性白色念珠菌感染模型。

Modeling of systemic candida albicans infec tion on immunosuppressed mice

Objective:To establish a murine model of systemic candidiasis by immunosuppressed mice for providing scientific basis for the research and treatment of infective diseases.Methods:Cyclophosphamide(CY) was given to the normal mice of Kunming species only once via abdominal cavity with a dosage of 200 mg/kg to observe its effects on the general state and leukocyte count in the mice.After that,the solution of candida albicans was administered intravenously in the mice via lateral tail vein with concentrations of 1.5×105/ml(low-concentration group),1.5×106/ml(mid-concentration group)or 1.5×107/ml(high-concentration group),respectively,to establish model of infection.Results: After injection of CY via abdominal cavity,the mice behaved quiet with occurrence of marasmus,reduced diet intake,polyuria,smoothless fur and reduced white cell count.When the above three groups were consecutively observed for three weeks,the mortality in the low-concentration group was 20% and 50% in the mid-concentration group,and that was 100% in the high concentration group.Tissue culture of organs of the mice confirmed that the internal organs were infected by C.albicans,which proved that the model of infection had been established successfully.Conclusion:Cyclophosphamide(CY) can decrease the number of leukouvely and result in immuno-suppression.The murine model of systemic candidiasis can be established by a suitable dose of C.albicans inoculated via lateral tail vein.