Interaction of isolated Lp(a) lipoprotein with calcium ions and glycosaminoglycans in vitro |
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| Authors: | C. Ericson,,G. Dahlé n, K. Berg |
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| Affiliation: | Laboratory of Clinical Chemistry, Central Hospital, Boden, Sweden;Institute of Medical Genetics, University of Oslo, Oslo, Norway |
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| Abstract: | The interaction between the lipoprotein carrying the Lp(a) antigen, i.e. the Lp(a) lipoprotein, and agarose gels substituted with glycosaminoglycans, as well as the precipitation of the Lp(a) lipoprotein by Ca++ were studied. Comparisons between Lp(a) lipoprotein and other serum lipoproteins were conducted.
Very low density lipoprotein (VLDL) and low density lipoprotein (LDL) were bound to the tested glycosaminoglycans at a low ionic strength of sodium chloride, but no binding was found with the Lp(a) lipoprotein. However, Ca++ as a divalent buffer cation gave a precipitating Ca++-Lp(a) lipoprotein complex even at a physiological Ca++ concentration. VLDL and LDL were not precipitated under these conditions. These findings may be of interest in relation to the previously reported higher frequency of the phenotype Lp(a+) in subjects with coronary heart disease. |
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